Determination of Fermentation of Four Novel Type 4 Resistant Starches by Sprague Dawley Male Rats (P20-006-19). (13th June 2019)

• Record Type: Journal Article
• Authors: Coulon, Diana; Page, Ryan; Guice, Justin; Raggio, Anne; Marx, Brian; Gourineni, Vishnupriya; Stewart, Maria; Keenan, Michael
• Is Part Of: Current developments in nutrition. Volume 3(2019)Supplement 1
• Publication Date: 2019-06-13
• Subjects: Nutrition -- Periodicals; Nutritional Physiological Phenomena; Nutrition; Periodicals; Periodicals; Fulltext; Internet Resources; Periodicals; 612.3
• DOI: 10.1093/cdn/nzz040.P20-006-19
• Languages: English
• ISSNs: 2475-2991

Abstract

Abstract: Objectives: The goal of this study was to determine fermentation of four novel resistant starch (RS) type 4 products (RSA, B, C, and D) in Sprague Dawley male rats compared to control (CON). Assessment was by standard measures: empty cecum weight (ECW), cecal contents weight (CCW), cecal contents pH (pH), cecal contents short-chain fatty acids: acetate, propionate and butyrate, cecal contents RS (CCRS), and serum active glucagon-like peptide 1 (GLP-1). Abdominal cavity fat (abfat)/body weight was determined. Methods: Forty weanling rats (Envigo), after a week quarantine, were randomly assigned to five groups ( n = 8) for a 3-week study. Starches (Ingredion Incorporated) were RS products as 10% of weight of RS diets (equivalent to human fiber requirement) and AMIOCA® starch (100% amylopectin) comprised 53.6% weight of CON and 43.6% weight of the RS diets (RSA – RSD). The RS products varied by % purity and origin (e.g., potato, tapioca, corn). Other ingredients (DYETS) were used (AIN-93 G). A one-way ANOVA was followed by Tukey post hoc test ( P < 0.05). At euthanasia, cecal contents, serum, GI tract, and abfat were collected. Emboweled body weight (EBW) was calculated as body weight minus GI contents. Results: RSB, RSC, and RSD demonstrated greater fermentation than CON and RSA. This was based on pH below 6.5 vs above 8.0, ECW above 0.7 g vs. below 0.54, CCW above 7.5 g vs below 3.86, and cecal contents acetate above 0.36 mmol/cecum vs below 0.21. Cecal contents propionate was increased for RSB and RSC (above 0.08 mmol/cecum vs below 0.06), but cecal contents butyrate and GLP-1 were not statistically significant. Post hoc power analysis for the latter two demonstrated power <80%. All groups had low CCRS. All four RS groups had lower abfat/EBW% than CON, but only RSA had a lower body weight. Conclusions: RSB, RSC, and RSD products were fermented; but it was inconclusive whether fermentation led to increased butyrate and serum GLP-1. Compared to CON all four novel RS groups had reduced abfat% indicating reduced energy density of the RS diets. Estimates of RS disappearance from cecum indicated digestion and/or fermentation of RS products with RSA apparently mainly digested. Funding Sources: Ingredion Incorporated, LSU AgCenter.