The Value of T-Cell Receptor γ (TRG) Clonality Evaluation by Next-Generation Sequencing in Clinical Hematolymphoid Tissues: A Descriptive Study of 41 Cases From a Single Institution. Issue 3 (5th July 2018)

• Record Type: Journal Article
• Authors: Kansal, Rina; Grody, Wayne W; Zhou, Jamie; Dong, Ling; Li, Xinmin
• Is Part Of: American journal of clinical pathology. Volume 150:Issue 3(2018)
• Page Start: 193
• Page End: 223
• Publication Date: 2018-07-05
• Subjects: Clonality -- T-cell receptor -- Next-generation sequencing -- Antigen receptor gene rearrangement -- PCR -- Lymphoproliferative disorder; Diagnosis, Laboratory -- Periodicals; Pathology -- Periodicals; 616.07
• DOI: 10.1093/ajcp/aqy046
• Languages: English
• ISSNs: 0002-9173

Abstract

Abstract: Objectives: To evaluate feasibility of assessing T-cell receptor γ ( TRG ) clonality by next-generation sequencing (NGS) in hematolymphoid tissues. Methods: We evaluated TRG clonality using NGS and polymerase chain reaction (PCR) assays in blood, bone marrow, and formalin-fixed, paraffin-embedded tissues in 41 archived cases, including 21 benign cases with no history of any lymphoproliferative disorders (LPDs), 16 LPDs (nine mature T-cell neoplasms, seven mature B-cell neoplasms and immune dysregulation–associated LPDs), and four atypical LPDs from 22 females and 19 males with a median age of 58 (range, 9-87) years. Results: (1) NGS analyzed TRG sequence and peak ratios, and it had a greater sensitivity than PCR. (2) NGS identified small clones, including biallelic or monoallelic, and minimum clonal percentages (range, ~2.4% to ~69%) within all T cells. (3) We provide our strategy and criteria for evaluating NGS results. (4) We describe every case, with definitive evaluation of TRG clonality in 100% cases by NGS. Conclusions: TRG clonality evaluation by NGS provides greater clinical utility than PCR.