Zinc source modulates intestinal inflammation and intestinal integrity of broiler chickens challenged with coccidia and Clostridium perfringens. (18th January 2019)

• Record Type: Journal Article
• Authors: Bortoluzzi, C; Lumpkins, B; Mathis, G F; França, M; King, W D; Graugnard, D E; Dawson, K A; Applegate, T J
• Is Part Of: Poultry science. Volume 98:Number 5(2019:May)
• Page Start: 2211
• Page End: 2219
• Publication Date: 2019-01-18
• Subjects: broiler -- coccidiosis -- necrotic enteritis -- zinc supplementation; Poultry -- Periodicals; Poultry -- Periodicals; Poultry Diseases -- Periodicals; Electronic journals; 636.5005
• DOI: 10.3382/ps/pey587
• Languages: English
• ISSNs: 0032-5791

Abstract

ABSTRACT: Two dietary sources of zinc (ZnSO4 or organic Zn) were tested in chickens challenged with coccidiosis (Co ) or coccidiosis plus Clostridium perfringens (CoCPF ). On day 14, the chickens were orally gavaged with ∼5, 000 Eimeria maxima sporulated oocysts. On day 19, 20, and 21 chickens challenged with C. perfringens were given a broth culture containing 10 8 cfu of this bacterium. Productive performance parameters were determined at d 14, 21, and 28. On day 21, necrotic enteritis (NE ) lesions were scored, and intestinal permeability was evaluated. Jejunum and cecal tonsils were collected for morphology and gene expression analysis. On day 21, organic Zn improved BW gain by 18.6% ( P = 0.07), and FCR by 12% ( P = 0.09) in CoCPF challenged chickens vs. birds fed ZnSO4 . From 1 to 28, organic Zn increased BW gain ( P = 0.02), and improved FCR ( P = 0.03) vs. birds fed ZnSO4 . At 21 d, NE lesions were only observed in CoCPF birds ( P < 0.001), and mortality due to NE was only observed when CoCPF birds were fed ZnSO4 ( P = 0.001). Organic Zn fed birds had increased villus height in the jejunum ( P = 0.005) and decreased intestinal permeability ( P = 0.01) vs. ZnSO4 . In the jejunum, organic Zn fed birds showed a downregulation of expression of IL-8 ( P = 0.02), and upregulation of IL-10 ( P = 0.05) in CoCPF birds vs. ZnSO4 - CoCPF birds. As main effect, birds supplemented with organic Zn had higher mRNA expression of TLR-2 ( P = 0.02) and IgA ( P = 0.01). In the cecal tonsils, organic Zn fed birds showed upregulation of iNOS ( P = 0.008) in CoCPF birds vs. ZnSO4 -CoCPF birds. Organic Zn supplementation reduced intestinal permeability and attenuated intestinal inflammation of broilers co-challenged with coccidia and C. perfringens .