Bacteriophage Therapy Increases Complement-Mediated Lysis of Bacteria and Enhances Bacterial Clearance After Acute Lung Infection With Multidrug-Resistant Pseudomonas aeruginosa. (22nd November 2018)

• Record Type: Journal Article
• Authors: Abd El-Aziz, Abeer M; Elgaml, Abdelaziz; Ali, Youssif M
• Is Part Of: Journal of infectious diseases. Volume 219:Number 9(2019)
• Page Start: 1439
• Page End: 1447
• Publication Date: 2018-11-22
• Subjects: Multidrug-resistant bacteria -- Pseudomonas aeruginosa -- lung infection -- bacteriophage -- phage therapy -- complement system; Communicable diseases -- Periodicals; Diseases -- Causes and theories of causation -- Periodicals; Medicine -- Periodicals; Communicable Diseases -- Periodicals; Electronic journals; 616.9
• DOI: 10.1093/infdis/jiy678
• Languages: English
• ISSNs: 0022-1899

Abstract

Abstract: Emergence of multidrug-resistant (MDR) bacterial infections is a major problem in clinical medicine. Development of new strategies such as phage therapy may be a novel approach for treatment of life-threatening infections caused by MDR bacteria. A newly isolated phage, MMI- Ps 1, with strong lytic activity was used for treatment of acute lung infection with Pseudomonas aeruginosa in a mouse model. Intranasal administration of a single dose of MMI- Ps 1 immediately after infection provided a significant level of protection and increased the survival duration. Moreover, treatment of infected mice with phage as late as 12 hours after infection was still protective. Our in vitro results are the first to show the synergistic elimination of serum-resistant Pseudomonas strains by phage and complement. Phage therapy increases the efficacy of complement-mediated lysis of serum-resistant P. aeruginosa strains, indicating the importance of an intact complement system in clearing Pseudomonas infection during phage therapy.