Effect of polyhexamethylene biguanide functionalized silver nanoparticles on the growth of Staphylococcus aureus. Issue 4 (19th February 2019)

• Record Type: Journal Article
• Authors: Yi, Jialin; Zhang, Yuelin; Lin, Wenshu; Niu, Bing; Chen, Qin
• Is Part Of: FEMS microbiology letters. Volume 366:Issue 4(2019)
• Publication Date: 2019-02-19
• Subjects: Staphylococcus aureus -- silver nanoparticles -- polyhexamethylene biguanide -- antibacterial activity -- membrane permeability -- membrane fluidity; Microbiology -- Periodicals; 579
• DOI: 10.1093/femsle/fnz036
• Languages: English
• ISSNs: 0378-1097

Abstract

ABSTRACT: Polyhexamethylene biguanide (PHMB) was used as a stabilizing ligand to synthesize uniform silver nanoparticles (Ag NPs). The effects and action mechanism of PHMB functionalized Ag NPs (Ag NPs-PHMB) on the growth of the Gram-positive bacteria Staphylococcus aureus were investigated. The results showed that a high concentration Ag NPs-PHMB could be obtained and prepared particles were fairly uniform. Prepared Ag NPs-PHMB enhanced the bactericidal effect and the log kill reached 5.06 when S. aureus was exposed to Ag NPs-PHMB for 20 min. Ag NPs-PHMB caused damage to cell wall, a decrease in the membrane fluidity, and leakage of K +, Mg 2+, ATP and proteins from the cell, eventually leading to the death of S. aureus . Abstract : Ag NPs-PHMB caused cell wall damage, decreased membrane fluidity, resulted in leakage of K +, Mg 2+, ATP and proteins from the cell, and inhibited DNA replication, eventually leading to the loss of viability and death of S. aureus .