Ten-day and four-week toxicity and toxicokinetics studies of alpha-glycosyl isoquercitrin in juvenile Göttingen minipigs. (11th June 2019)

• Record Type: Journal Article
• Authors: Maronpot, Robert R; Ramot, Yuval; Koyanagi, Mihoko; Dias, Nicola; Cameron, David; Eniola, Samuel; Nyska, Abraham; Hayashi, Shim-mo
• Is Part Of: Toxicology research and application. Volume 3(2019)
• Publication Date: 2019-06-11
• Subjects: Alpha-glycosyl isoquercitrin -- isoquercitrin -- toxicity -- safety -- AGIQ -- Göttingen minipigs; Toxicology -- Periodicals; 571.9505
• DOI: 10.1177/2397847319855087
• Languages: English
• ISSNs: 2397-8473

Abstract

Background: Alpha-glycosyl isoquercitrin (AGIQ) is widely used as an anti-oxidative food additive in many products. Nevertheless, information on its safety and toxicity is still very limited. A 90-day toxicity study in rats and comprehensive genotoxic studies have proven AGIQ to be safe. Aim: The goal of this study was to assess the safety of use of AGIQ in infant formula. To test for potential adverse effects on growth or other safety issues specific for young animals, we performed a 10-day and a 4-week study in preweaning Göttingen minipigs. Method: Newborn minipigs were treated four times per day with oral AGIQ (0, 100, 300, or 1000 mg/kg/day) for 10 days or 4 weeks. Results: All animals remained in good health throughout both studies, and there were no treatment-related signs of toxicity or abnormal findings in blood parameters. In the 4-week study, yellow coloration of the bones was seen in all animals in the high-dose group with no related histological findings. Hepatocellular and sinusoidal/Kupffer cell iron deposition was seen in the liver of all animals in both studies, as expected after the routine administration of supplemental iron to newborn animals. There was sufficient evidence of systemic exposure based on plasma levels of AGIQ metabolites. Conclusion: Taken together, these findings show that oral administration of AGIQ in reconstituted milk supplement to preweaning Göttingen minipigs for 4 weeks at up to 1000 mg/kg/day does not result in any adverse treatment-related effects and further support the safety of AGIQ as a food additive.