Metabolic coupling in the co-cultured fungal-yeast suite of Trametes ljubarskyi and Rhodotorula mucilaginosa leads to hypersecretion of laccase isozymes. Issue 12 (December 2019)

• Record Type: Journal Article
• Authors: Kumar, Amit; Arora, Sakshi; Jain, Kavish Kumar; Sharma, Krishna Kant
• Is Part Of: Fungal biology. Volume 123:Issue 12(2019)
• Page Start: 913
• Page End: 926
• Publication Date: 2019-12
• Subjects: Antioxidants -- Co-cultivation -- Dye-decolorization -- Oxidoreductase -- Proteome -- Scanning electron microscopy; Mycology -- Periodicals; Fungi -- Periodicals; 579.505
• DOI: 10.1016/j.funbio.2019.09.013
• Languages: English
• ISSNs: 1878-6146

Abstract

Abstract: Trametes ljubarskyi produces multiple laccase isozymes under various physicochemical conditions. During co-cultivation condition Rhodotorula mucilaginosa showed inter-specific interactions with T. ljubarskyi and hypersecretion of laccases; however, the underlying molecular mechanism is less-known. The analysis of proteomics data of co-cultivated cultures revealed the mechanism of metabolic coupling during fungal-yeast interactions. The results suggested high score GO terms related to stimulus-response, protein binding, membrane components, transport channels, oxidoreductases, and antioxidants. The SEM studies confirmed the cellular communication and their inter-specific interactions. This study allows us to deepen and refine our understanding of fungal-yeast symbiotic interaction; further, it also establishes a mutual relation by metabolic coupling for 10-fold higher laccase isozyme secretion (6532 U/ml). The purified laccase isozymes showed acidic pH optima (pH 3–4), higher thermo-stability (60 °C), and broad enzyme kinetics ( K m ) values. Our study also provides an in-depth understanding of laccase isozymes and their potential to degrade synthetic dyes, which may help the fungi to survive in an adverse environment. Highlights: R hodotorula mucilaginosa stimulate laccase isozymes from T rametes ljubarskyi in co-culture. Proteomic study suggests increase in stimulus-response, antioxidant and oxidoreductases. SEM analysis showed inter-specific interaction between mycelial fungi and yeast. Malachite green was completely decolorized by laccase isozymes from co-cultivation.