In Vivo Mutagenicity Testing of Arylboronic Acids and Esters. (17th August 2019)

• Record Type: Journal Article
• Authors: Masuda‐Herrera, Melisa J.; Dobo, Krista L.; Kenyon, Michelle O.; Kenny, Julia D.; Galloway, Sheila M.; Escobar, Patricia A.; Reddy, M. Vijayaraj; Jolly, Robert A.; Trejo‐Martin, Alejandra; Brown, Caren; Mckeon, Marie; Young, Megan; Bruce, Shannon; Pant, Kamala; Dutta, Aparajita; Kulkarni, Rohan; Bercu, Joel P.
• Is Part Of: Environmental and molecular mutagenesis. Volume 60:Number 9(2019)
• Page Start: 766
• Page End: 777
• Publication Date: 2019-08-17
• Subjects: Pig‐a -- micronucleus -- comet -- impurity; Mutagenesis -- Periodicals; Molecular genetics -- Periodicals; Mutagenèse -- Périodiques; Mutagenèse chimique -- Périodiques; Mutation -- Périodiques; Maladies de l'environnement -- Périodiques; Génétique moléculaire -- Périodiques; 576.542
• DOI: 10.1002/em.22320
• Languages: English
• ISSNs: 0893-6692

Abstract

Abstract : Arylboronic acids and esters (referred to collectively as arylboronic compounds) are commonly used intermediates in the synthesis of pharmaceuticals but pose a challenge for chemical syntheses because they are often positive for bacterial mutagenicity in vitro . As such, arylboronic compounds are then typically controlled to levels that are acceptable for mutagenic impurities, that is, the threshold of toxicological concern (TTC). This study used ICH M7 guidance to design and conduct a testing strategy to investigate the in vivo relevance of the in vitro positive findings of arylboronic compounds. Eight arylboronic compounds representing a variety of chemical scaffolds were tested in Sprague Dawley and/or Wistar rats in the in vivo Pig‐a (peripheral blood reticulocytes and mature red blood cells) and/or comet assays (duodenum and/or liver). Five of the eight compounds were also tested in the micronucleus (peripheral blood) assay. The arylboronic compounds tested orally demonstrated high systemic exposure; thus the blood and bone marrow were adequately exposed to test article. One compound was administered intravenously due to formulation stability issues. This investigation showed that arylboronic compounds that were mutagenic in vitro were not found to be mutagenic in the corresponding in vivo assays. Therefore, arylboronic compounds similar to the scaffolds tested in this article may be considered non‐mutagenic and managed in accordance with the ICH Q3A/Q3B guidelines. Environ. Mol. Mutagen. 2019. © 2019 Wiley Periodicals, Inc.