AH2 encodes a MYB domain protein that determines hull fate and affects grain yield and quality in rice. (3rd September 2019)

• Record Type: Journal Article
• Authors: Ren, Deyong; Cui, Yuanjiang; Hu, Haitao; Xu, Qiankun; Rao, Yuchun; Yu, Xiaoqi; Zhang, Yu; Wang, Yuexing; Peng, Youlin; Zeng, Dali; Hu, Jiang; Zhang, Guangheng; Gao, Zhenyu; Zhu, Li; Chen, Guang; shen, Lan; Zhang, Qiang; Guo, Longbiao; Qian, Qian
• Is Part Of: Plant journal. Volume 100:Number 4(2019)
• Page Start: 813
• Page End: 824
• Publication Date: 2019-09-03
• Subjects: rice (Oryza sativa) -- AH2 -- hull fate -- grain yield and quality -- CRISPR‐Cas9 -- transcriptional repressor; Plant molecular biology -- Periodicals; Plant cells and tissues -- Periodicals; Botany -- Periodicals; 580
• DOI: 10.1111/tpj.14481
• Languages: English
• ISSNs: 0960-7412

Abstract

Significance Statement: AH2 plays an important role in the determination of hull epidermis development, palea identity, and grain size. Summary: The palea and lemma (hull) are grass‐specific organs, and determine grain size and quality. In the study, AH2 encodes a MYB domain protein, and functions in the development of hull and grain. Mutation of AH2 produces smaller grains and alters grain quality including decreased amylose content and gel consistency, and increased protein content. Meantime, part of the hull lost the outer silicified cells, and induces a transformation of the outer rough epidermis to inner smooth epidermis cells, and the body of the palea was reduced in the ah2 mutant. We confirmed the function of AH2 by complementation, CRISPR‐Cas9, and cytological and molecular tests. Additionally, AH2, as a repressor, repress transcription of the downstream genes. Our results revealed that AH2 plays an important role in the determination of hull epidermis development, palea identity, and grain size.