In vivo monitoring of thrombosis in mice by optical coherence tomography. Issue 11 (5th August 2019)

• Record Type: Journal Article
• Authors: Yu, Yao; Yu, Menghan; Liu, Jian; Ding, Ning; Huang, Jiangmei; Wan, Dong; Zhao, Yuliang; Ma, Zhenhe
• Is Part Of: Journal of biophotonics. Volume 12:Issue 11(2019)
• Page Start: n/a
• Page End: n/a
• Publication Date: 2019-08-05
• Subjects: antithrombotic drugs -- mice -- optical coherence tomography -- thrombosis; Photonics -- Periodicals; Optical materials -- Periodicals; Optics -- Periodicals; Medical instruments and apparatus -- Periodicals; 621.3605
• DOI: 10.1002/jbio.201900105
• Languages: English
• ISSNs: 1864-063X

Abstract

Abstract: The objective of this study is to establish a novel method for continuously monitoring thrombus progression with various outcome measures and to assess the efficacy of antithrombotic drugs in murine thrombosis model in mice. In the study, thrombus was induced in the femoral vein of mice by FeCl3 and monitored over time by spectral‐domain optical coherence tomography (OCT). Three‐dimensional images of thrombi with or without heparin as an antithrombotic agent were obtained from OCT angiography. In addition, several parameters of thrombi were analyzed and compared between control and anticoagulant groups. By using OCT, we were able to trace thrombus generation in the same mouse in real time. We found that in our model heparin reduced thrombus size by ~60% and thrombus cross‐sectional area by 50%. OCT results also show that both time to thrombus size (>0.02mm 3 ) and time to occlusion (>30%) were significantly reduced after heparin addition. This study demonstrates that OCT reliably monitors thrombus generation and progression from various aspects including thrombus size. This enables us to measure the kinetic of thrombosis more accurately, and effectively evaluate the efficacy and activities of antithrombotic drugs. This model may represent a useful tool in antithrombotic drug discoveries in preclinical studies. Abstract : Thrombosis monitoring in vivo in small animals is of great value in basic research. The aim of this study is to utilize OCT to monitor thrombosis progression in femoral vein of mice from various measurement criteria, and to validate its use in evaluation the efficacy of the antithrombotic drug. The proved capability of obtaining thrombodynamics information in mice model provides valuable use in preclinical studies for anti‐thrombotic drugs development research.