Efficacy of copolymer scaffolds delivering human demineralised dentine matrix for bone regeneration. (May 2019)

• Record Type: Journal Article
• Authors: Munir, Arooj; Døskeland, Anne; Avery, Steven J; Fuoco, Tiziana; Mohamed-Ahmed, Samih; Lygre, Henning; Finne-Wistrand, Anna; Sloan, Alastair J; Waddington, Rachel J; Mustafa, Kamal; Suliman, Salwa
• Is Part Of: Journal of tissue engineering. Volume 10(2019)
• Publication Date: 2019-05
• Subjects: Growth factor -- mesenchymal stem cell -- bone tissue engineering -- drug delivery -- functionalisation; Tissue engineering -- Periodicals; 612.028
• DOI: 10.1177/2041731419852703
• Languages: English
• ISSNs: 2041-7314

Abstract

Poly(L-lactide-co-ε-caprolactone) scaffolds were functionalised by 10 or 20 µg/mL of human demineralised dentine matrix. Release kinetics up to 21 days and their osteogenic potential on human bone marrow stromal cells after 7 and 21 days were studied. A total of 390 proteins were identified by mass spectrometry. Bone regeneration proteins showed initial burst of release. Human bone marrow stromal cells were cultured on scaffolds physisorbed with 20 µg/mL and cultured in basal medium (DDM group) or physisorbed and cultured in osteogenic medium or cultured on non-functionalised scaffolds in osteogenic medium. The human bone marrow stromal cells proliferated less in demineralised dentine matrix group and activated ERK/1/2 after both time points. Cells on DDM group showed highest expression of IL-6 and IL-8 at 7 days and expressed higher collagen type 1 alpha 2, SPP1 and bone morphogenetic protein-2 until 21 days. Extracellular protein revealed higher collagen type 1 and bone morphogenetic protein-2 at 21 days in demineralised dentine matrix group. Cells on DDM group showed signs of mineralisation. The functionalised scaffolds were able to stimulate osteogenic differentiation of human bone marrow stromal cells.