Sequencing-Based Transcriptome-Wide Targeted Genotyping for Evolutionary and Ecological Studies. (March 2019)

• Record Type: Journal Article
• Authors: Zhu, Xuan; Wang, Jing; Lv, Jia; Liu, Pingping; Zhang, Lingling; Jiao, Wenqian; Ma, Cen; Bao, Zhenmin; Wang, Shi
• Is Part Of: Evolutionary bioinformatics online. Volume 15(2019)
• Publication Date: 2019-03
• Subjects: HD-Marker -- transcriptome-wide targeted genotyping -- molecular markers -- non-model organism; Bioinformatics -- Periodicals; Evolutionary computation -- Periodicals; Genetic programming (Computer science) -- Periodicals; Computational Biology; Evolution, Molecular; Bioinformatics; Electronic journals; Periodicals; Fulltext; Internet Resources; Periodicals; Periodicals; 576.8
• DOI: 10.1177/1176934319836074
• Languages: English
• ISSNs: 1176-9343

Abstract

Transcriptome-wide targeted genotyping is highly attractive for evolutionary and ecological studies but, until recently, accomplishing this goal presented a major technical barrier for the study of non-model organisms. Our group has recently developed a high-throughput targeted genotyping approach (called HD-Marker) based on the high specificity and accuracy of oligo extension-ligation assays that facilitates the design of assays tailored to meet specific genotyping needs. HD-Marker allows for targeted genotyping of over 10 000 genes in a single tube, with strikingly high capture rate (98%-99%) and genotyping accuracy (97%-99%). With the remarkable advantages of cost-effectiveness and flexibility, we envision that HD-Marker has broad application potential in evolutionary and ecological studies.