Differential distributions of trafficking and signaling proteins of the maize ER-Golgi apparatus. (2nd December 2019)

• Record Type: Journal Article
• Authors: Okekeogbu, Ikenna O.; Aryal, Uma K.; Fernández-Niño, Susana M. González; Penning, Bryan W.; Heazlewood, Joshua L.; McCann, Maureen C.; Carpita, Nicholas C.
• Is Part Of: Plant signaling & behavior. Volume 14:Number 12(2019)
• Publication Date: 2019-12-02
• Subjects: Maize (Zea mays) -- Endoplasmic reticulum (ER) -- Golgi -- Small GTPase Rab proteins -- 14-3-3 proteins -- Calcium-dependent proteins -- Cellulose synthase (CesA); Plant ecophysiology -- Periodicals; Plant cellular signal transduction -- Periodicals; Plant cellular signal transduction; Plant ecophysiology; Periodicals; Electronic journals; 581
• DOI: 10.1080/15592324.2019.1672513
• Languages: English
• ISSNs: 1559-2316

Abstract

ABSTRACT: The Endoplasmic Reticulum (ER)-Golgi apparatus of plants is the site of synthesis of non-cellulosic polysaccharides that then traffic to the cell wall. A two-step protocol of flotation centrifugation followed by free-flow electrophoresis (FFE) resolved ER and Golgi proteins into three profiles: an ER-rich fraction, two Golgi-rich fractions, and an intermediate fraction enriched in cellulose synthases. Nearly three dozen Rab-like proteins of eight different subgroups were distributed differentially in ER- vs. Golgi-rich fractions, whereas seven 14-3-3 proteins co-fractionated with cellulose synthases in the intermediate fraction. FFE offers a powerful means to classify resident and transient proteins in cell-free assays of cellular location.