A fluorescent nanoparticle probe based on sugar-substituted tetraphenylethene for label-free detection of galectin-3. Issue 43 (24th October 2019)

• Record Type: Journal Article
• Authors: Zhang, Zhiling; Yu, Yong; Zhao, Yueyue; Ng, Ka Ming
• Is Part Of: Journal of materials chemistry. Volume 7:Issue 43(2019)
• Page Start: 6737
• Page End: 6741
• Publication Date: 2019-10-24
• Subjects: Materials -- Periodicals; Chemistry, Analytic -- Periodicals; Biomedical materials -- Research -- Periodicals; 543.0284
• DOI: 10.1039/c9tb01703h
• Languages: English
• ISSNs: 2050-750X

Abstract

Abstract : An AIE-active nanoparticle probe decorated with galactoside residues is designed and developed for label-free detection of galectin-3 with enhanced specificity, showing promising applications in cancer diagnoses. Abstract : Galectin-3 is a vital biomarker for cancer progression. An aggregation-induced emission (AIE)-active nanoparticle probe is designed and synthesized for label-free detection of galectin-3 based on carbohydrate–protein interactions. The probe employs tetraphenylethene (TPE) as the fluorescent core structure and N -acetyllactosamine (LacNAc) as galactoside residues and self-aggregates into uniform nanoparticles with multiple binding sites on the surface targeting galectin-3. Upon crosslinking of the nanoparticles and galectin-3, large complexes are formed and a significant fluorescence change is measured, allowing rapid, sensitive and specific detection of galectin-3 via a fluorometric assay. Its demonstrated superior anti-interference ability and applicability in serum samples offer promising applications in cancer diagnoses.