Novel nanoparticles of cerium-doped zeolitic imidazolate frameworks with dual benefits of antibacterial and anti-inflammatory functions against periodontitis. Issue 44 (16th October 2019)

• Record Type: Journal Article
• Authors: Li, Xue; Qi, Manlin; Li, Chunyan; Dong, Biao; Wang, Jia; Weir, Michael D.; Imazato, Satoshi; Du, Liuyi; Lynch, Christopher D.; Xu, Lin; Zhou, Yanmin; Wang, Lin; Xu, Hockin H. K.
• Is Part Of: Journal of materials chemistry. Volume 7:Issue 44(2019)
• Page Start: 6955
• Page End: 6971
• Publication Date: 2019-10-16
• Subjects: Materials -- Periodicals; Chemistry, Analytic -- Periodicals; Biomedical materials -- Research -- Periodicals; 543.0284
• DOI: 10.1039/c9tb01743g
• Languages: English
• ISSNs: 2050-750X

Abstract

Abstract : Novel ZIF-8:Ce nanoparticles provide a unique insight into effective anti-inflammatory and antibacterial platforms for periodontitis treatment. Abstract : Periodontitis is a common bacteria-borne inflammatory disease that can damage the supporting structures of teeth, eventually leading to tooth loss and systemic inflammations. The present study developed novel nanoparticles (ZIF-8:Ce) by doping cerium (Ce) into zeolitic imidazolate framework-8 (ZIF-8) to simultaneously obtain antibacterial and anti-inflammatory capabilities. The objectives of this study were to: (1) develop novel ZIF-8 nanoparticles doped with different Ce/(Ce + Zn) molar ratios of 1%, 5% and 10% to combat periodontitis; (2) investigate the inhibition efficacy of different nanoparticles against biofilms of periodontal pathogens; and (3) evaluate the effects of different ZIF-8:Ce on inflammatory secretion and macrophage polarization in vitro . The results showed that Ce doping at a ratio from 1% to 10% did not compromise the regular and uniform structure of ZIF-8. ZIF-8:Ce possessed a sustained Zn 2+ and Ce 3+ /Ce 4+ release and favorable superoxide dismutase/catalase activities without cytotoxicity at a concentration below 30 μg mL −1 . ZIF-8 exhibited an excellent anti-biofilm function against periodontal pathogens. Although 10% Ce doping into ZIF-8 slightly reduced the antibacterial effects, the CFU reduction still remained at approximately 2 orders of magnitude. More importantly, ZIF-8:Ce exhibited increasing anti-inflammatory effects with increasing amounts of Ce doping. In addition, ZIF-8:Ce10% exerted much better anti-inflammatory effects by inhibiting pro-inflammatory factor expression via restraining the NF-kB/p65 subunit translocation ( p < 0.05). Meanwhile, ZIF-8:Ce10% elevated the polarization of the M2 phenotype of macrophages and promoted anti-inflammatory cytokine secretion. Therefore, the novel ZIF-8:Ce nanoparticles provided a unique insight into the development of effective anti-inflammatory and antibacterial platforms for treating periodontitis.