Defect-related luminescent nanostructured hydroxyapatite promotes mineralization through both intracellular and extracellular pathways. Issue 62 (5th November 2019)

• Record Type: Journal Article
• Authors: Dai, Chunyan; Zhu, Linhua; Chen, Guangying; Haddleton, David M.
• Is Part Of: RSC advances. Volume 9:Issue 62(2019)
• Page Start: 35939
• Page End: 35947
• Publication Date: 2019-11-05
• Subjects: Chemistry -- Periodicals; 540.5
• DOI: 10.1039/c9ra06629b
• Languages: English
• ISSNs: 2046-2069

Abstract

Abstract : Hydroxyapatite (HAP) is a widely used biomaterial for bone tissue substitution due to its chemical similarity with the natural bone. Abstract : Hydroxyapatite (HAP) is a widely used biomaterial for bone tissue substitution due to its chemical similarity with the natural bone. Defect-related luminescent HAP materials have the same chemical composition as normal HAP and excellent biocompatibility. However, only few works have focused on the defect-related luminescent HAP materials on bone regeneration. In this work, we systematically investigated the bone regeneration pathway induced by nanostructured particles using defect-related luminescent hydroxyapatite (S2) materials. We monitored the subcellular distribution and location of S2 during osteoblast differentiation with the property of defect-related luminescence. Nano-scale S2 could be internalized by osteoblasts (OBs) via caveolae-mediated endocytosis and macropinocytosis. S2 incorporated into the lysosomes dissolved and released calcium ions for the formation of mineralized nodules. Extracellular S2 also promoted bone regeneration as a nucleation site. Taken together, the physical properties of hydroxyapatite control the bone regeneration pathway in osteoblasts.