Digital quantitative detection of serum circulating miRNAs using dual-enhanced magnetobiosensors based on cascaded nucleic acid circuits. Issue 91 (29th October 2019)

• Record Type: Journal Article
• Authors: Liu, Chunxue; An, Yayun; Zhang, Yuanfu; Li, Xia; Xue, Qingwang; Wang, Huaisheng
• Is Part Of: Chemical communications. Volume 55:Issue 91(2019)
• Page Start: 13733
• Page End: 13736
• Publication Date: 2019-10-29
• Subjects: Chemistry -- Periodicals; 540
• DOI: 10.1039/c9cc07841j
• Languages: English
• ISSNs: 1359-7345

Abstract

Abstract : Here, we developed a dual-enhanced magnetobiosensor based on cascaded nucleic acid circuits for sensitive, portable and digital quantitative detection of circulating miRNAs in serum by a personal glucose meter (PGM). Abstract : The tough challenges for the convenient and quantitative determination of circulating miRNAs (cmiRNAs) in the peripheral blood are low abundance, high interference and lack of direct digital readout. Here, we developed dual-enhanced magnetobiosensors based on cascaded nucleic acid circuits, which integrate catalyzed hairpin assembly (CHA) with the hybridization chain reaction (HCR), for sensitive, portable and digital quantitative detection of circulating miRNAs in serum by a personal glucose meter (PGM).