Direct fluorescent glycan labeling with recombinant sialyltransferases. (30th July 2019)

• Record Type: Journal Article
• Authors: Wu, Zhengliang L; Person, Anthony D; Burton, Andrew J; Singh, Ravinder; Burroughs, Barbara; Fryxell, Dan; Tatge, Timothy J; Manning, Timothy; Wu, Guoping; Swift, Karl A D; Kalabokis, Vassili
• Is Part Of: Glycobiology. Volume 29:Number 11(2019)
• Page Start: 750
• Page End: 754
• Publication Date: 2019-07-30
• Subjects: enzymatic labeling -- fluorescent labeling -- glycan labeling -- sialic acid -- sialyltransferase; Glycoproteins -- Periodicals; Glycolipids -- Periodicals; Glycoconjugates -- Periodicals; 572.567
• DOI: 10.1093/glycob/cwz058
• Languages: English
• ISSNs: 0959-6658

Abstract

Abstract: Glycosylation is a common modification found on numerous proteins and lipids. However, direct detection of glycans on these intact biomolecules has been challenge. Here, utilizing enzymatic incorporation of fluorophore-conjugated sialic acids, dubbed as direct fluorescent glycan labeling, we report the labeling and detection of N- and O-glycans on glycoproteins. The method allows detection of specific glycans without the laborious gel blotting and chemiluminescence reactions used in Western blotting. The method can also be used with a variety of fluorescent dyes.