Potassium iodide and miltefosine inhibit biofilms of Sporothrix schenckii species complex in yeast and filamentous forms. (21st November 2018)

• Record Type: Journal Article
• Authors: Brilhante, Raimunda Sâmia Nogueira; Silva, Maria Lucilene Queiroz da; Pereira, Vandbergue Santos; de Oliveira, Jonathas Sales; Maciel, Juliana Maria; Silva, Isaac Neto Goes da; Garcia, Lana Glerieide Silva; Guedes, Glaucia Morgana de Melo; Cordeiro, Rossana de Aguiar; Pereira-Neto, Waldemiro de Aquino; de Camargo, Zoilo Pires; Rodrigues, Anderson Messias; Sidrim, José Júlio Costa; Castelo-Branco, Débora de Souza Collares Maia; Rocha, Marcos Fábio Gadelha
• Is Part Of: Medical mycology. Volume 57:Number 6(2019)
• Page Start: 764
• Page End: 772
• Publication Date: 2018-11-21
• Subjects: Sporothrix -- potassium iodide -- miltefosine -- susceptibility -- biofilm; Medical mycology -- Periodicals; Veterinary mycology -- Periodicals; Mycology -- Periodicals; Mycoses -- Periodicals; Pathogenic fungi -- Periodicals; 616.969005
• DOI: 10.1093/mmy/myy119
• Languages: English
• ISSNs: 1369-3786

Abstract

Abstract: This study aimed to evaluate the yeast biofilm growth kinetics and ultrastructure of Sporothrix schenckii complex and assess their mature biofilm susceptibility in filamentous and yeast forms to potassium iodide (KI) and miltefosine (MIL). Yeast biofilms were evaluated by crystal violet staining, XTT reduction assay and microscopic techniques. Susceptibility of planktonic and sessile cells was analyzed by broth microdilution. S. schenckii complex in yeast form produced biofilms, with an optimum maturation at 96 h, showing multilayered blastoconidia embedded in extracellular matrix. KI and MIL minimum inhibitory concentration (MIC) ranges against planktonic cells were 62, 500–250, 000 μg/ml and 0.125–4 μg/ml, respectively. KI and MIL reduced biofilm metabolic activity by 75.4% and 67.7% for filamentous form and 55.1% and 51.6% for yeast form, respectively. This study demonstrated that S. schenckii complex forms biofilms in vitro, and potassium iodide and miltefosine inhibit Sporothrix spp. biofilms in both filamentous and yeast forms.