Preparation and effect of lyophilized platelet-rich fibrin on the osteogenic potential of bone marrow mesenchymal stem cells in vitro and in vivo. Issue 10 (October 2019)

• Record Type: Journal Article
• Authors: Wang, Zhifa; Han, Leng; Sun, Tianyu; Wang, Weijian; Li, Xiao; Wu, Buling
• Is Part Of: Heliyon. Volume 5:Issue 10(2019)
• Publication Date: 2019-10
• Subjects: Bioengineering -- Cell biology -- Materials science -- Molecular biology -- Stem cell research -- Lyophilized platelet-rich fibrin -- Bone marrow mesenchymal stem cells -- Cell sheet -- Bone tissue engineering; Research -- Periodicals; Medical sciences -- Periodicals; Natural history -- Periodicals; Social sciences -- Periodicals; Earth sciences -- Periodicals; Physical sciences -- Periodicals; 507.2
• DOI: 10.1016/j.heliyon.2019.e02739
• Languages: English
• ISSNs: 2405-8440

Abstract

Abstract: Objectives: The goal of this study was to prepare lyophilized platelet-rich fibrin (L-PRF) and analyze the combined use of L-PRF and osteogenic bone marrow mesenchymal stem cell (BMSC) sheet fragments for bone tissue engineering via in vivo injection. Methods: First, fresh PRF (F-PRF) was lyophilized to prepare L-PRF, the characteristics of which were examined through gross morphological, and histological and microstructural observations. In addition, the kinetics of growth factor release from L-PRF and F-PRF were also determined by enzyme-linked immunosorbent assay (ELISA). Subsequently, after assessing the proliferation and osteogenic differentiation of BMSCs exposed to L-PRF or F-PRF in vitro, we subcutaneously injected BMSC sheet fragments with L-PRF or F-PRF into nude mice and assessed bone formation through microcomputed tomography and histological analyses. Results: We observed that L-PRF released growth factors that favored BMSC proliferation and osteogenic differentiation in vitro. The combined use of L-PRF and osteogenic BMSC sheet fragments enabled bone tissue regeneration in vivo, and no significant difference between the F-PRF and L-PRF groups was observed (P = 0.24). Conclusions: The results of this study demonstrate that the combined use of L-PRF and osteogenic BMSC sheets may have potential in the fabrication of engineered bone. Abstract : Bioengineering; Cell biology; Materials science; Molecular biology; Stem cell research; lyophilized platelet-rich fibrin; bone marrow mesenchymal stem cells; cell sheet; bone tissue engineering.