A New Mixed‐Ligand Coordination Polymer: Crystal Structure, Molecular Docking and Alleviating Effect on Myocarditis by Inhibiting the NLRP3‐Caspase‐1 Pathway in Cardiomyocytes. Issue 39 (21st October 2019)

• Record Type: Journal Article
• Authors: Li, Xiaozheng; Jin, Xiaowan; Li, Baoyi; Dong, Fengquan; Li, Haiying
• Is Part Of: ChemistrySelect. Volume 4:Issue 39(2019)
• Page Start: 11500
• Page End: 11505
• Publication Date: 2019-10-21
• Subjects: Coordination polymer -- Co(II)-complex -- mixed-ligand -- molecular docking -- myocarditis; Chemistry -- Periodicals; 540.5
• DOI: 10.1002/slct.201901797
• Languages: English
• ISSNs: 2365-6549

Abstract

Abstract: A new mixed‐ligand coordination polymer {[Co(Hbidc)(2, 2′‐bpy)(H2 O)2 ]⋅ 1.5H2 O}n (1 ) has been successfully prepared by the reaction of Co(NO3 )2 ⋅6H2 O, 2, 2′‐bipyridine (2, 2′‐bpy) as well as benzimidazole‐5, 6‐dicarboxylic acid (H3 bidc) in the mixed solvent of water and DMA under solvothermal conditions. Furthermore, a mechanical grinding method was used to produce the nanostructure 1 with the average size of 263 nm. The integrity of H9 C2 cardiomyocytes cell membrane after nano 1 treatment was detected by LDH detection. After nano 1 incubation, the inflammatory level in the H9 C2 cardiomyocytes was evaluated by western blot and ELISA measurement. In addition, the ROS accumulation in H9 C2 cardiomyocytes after nano 1 treatment was determined by H2DCFDA detection assay. Finally, possible interactions between the studied complex 1 and protein has been probed using molecular docking simulation. Abstract : A new Co(II)‐based coordination polymer as well as its nanoparticle have been prepared. The integrity of H9 C2 cardiomyocytes cell membrane after compound treatment was detected by LDH (lactate dehydrogenase) detection. After compound incubation, the inflammatory level in the H9 C2 cardiomyocytes was evaluated by western blot and ELISA (enzyme‐linked immunosorbent assay) measurement. In addition, the ROS (Reactive oxygen species) accumulation in H9 C2 cardiomyocytes after compound treatment was determined by H2 DCFDA (2‐(3, 6‐Diacetoxy‐2, 7‐dichloro‐9H‐xanthen‐9‐yl)benzoic acid) detection assay. Finally, possible interactions between the studied compound and protein has been probed using molecular docking simulation.