Analysis of miRNA‐mRNA regulatory network revealed key genes induced by aflatoxin B1 exposure in primary human hepatocytes. Issue 11 (9th September 2019)

• Record Type: Journal Article
• Authors: Zhang, Ziyang; Tang, Dongyang; Wang, Bin; Wang, Zhiwei; Liu, Mingjiu
• Is Part Of: Molecular genetics & genomic medicine. Volume 7:Issue 11(2019)
• Page Start: n/a
• Page End: n/a
• Publication Date: 2019-09-09
• Subjects: aflatoxin B1 -- hepatocellular carcinoma -- miRNA/mRNA regulatory network; Medical genetics -- Periodicals; Genomics -- Periodicals; 616.042
• DOI: 10.1002/mgg3.971
• Languages: English
• ISSNs: 2324-9269

Abstract

Abstract: Background: Aflatoxin B1 (AFB1) exposure is a crucial factor to initiate hepatocellular carcinoma (HCC). However, comprehensive microRNA (miRNA)‐message RNA (mRNA) regulatory network regarding AFB1‐associated HCC is still lacking. This work was aimed to identify miRNA‐mRNA network in primary human hepatocytes after AFB1 exposure. Methods: A miRNA expression dataset GSE71540 obtained from the gene expression omnibus (GEO) was used to identify differentially expressed miRNAs (DEMs) after AFB1 exposure using GEO2R. Target genes of these DEMs were identified using TargetScan V_7.2, miRDB, PITA, miRanda, and miRTarBase. Gene ontology (GO) annotation and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analyses were performed at Database for Annotation, Visualization and Integrated Discovery (DAVID). miRNA‐mRNA regulatory network was established by analyzing three enriched KEGG pathways significantly correlated with HCC onset and then visualized at CytoScape. Results: In this work, nine upregulated and nine downregulated DEMs were identified. Functional enrichment analyses showed that these predicted target genes were significantly associated with cancer development. Analysis of three enriched pathways related to the onset of HCC identified 13 and nine target genes for upregulated DEMs and downregulated DEMs, respectively. Subsequently, the miRNA‐mRNA regulatory networks were constructed. Conclusions: In conclusion, miRNA‐mRNA regulatory network was established, which will help to understand the mechanism underlying the AFB1‐induced onset of HCC. Abstract : MicroRNA (miRNA) and message RNA (mRNA) related to aflatoxin B1 (AFB1) exposure was identified. miRNA‐mRNA network related to AFB1‐induced hepatocellular carcinoma initiation was reported.