Trp‐1999‐Ser mutation of acetyl‐CoA carboxylase and cytochrome P450s‐involved metabolism confer resistance to fenoxaprop‐P‐ethyl in Polypogon fugax. Issue 12 (13th May 2019)

• Record Type: Journal Article
• Authors: Zhao, Ning; Ge, Lu'an; Yan, Yanyan; Bai, Shuang; Wang, Dandan; Liu, Weitang; Wang, Jinxin
• Is Part Of: Pest management science. Volume 75:Issue 12(2019)
• Page Start: 3175
• Page End: 3183
• Publication Date: 2019-05-13
• Subjects: acetyl‐CoA carboxylase (ACCase) -- multiple alleles -- metabolic resistance -- non‐target‐site resistance (NTSR) -- cytochrome P450s -- target‐site resistance (TSR); Pests -- Control -- Periodicals; Pesticides -- Periodicals; 632.9
• DOI: 10.1002/ps.5435
• Languages: English
• ISSNs: 1526-498X

Abstract

Abstract: BACKGROUND: Asia minor bluegrass ( Polypogon fugax Nees ex Steud.) is an invasive grass species severely infesting wheat and canola fields in China. In May 2017, a suspected resistant P. fugax population AHHY that survived fenoxaprop‐ P ‐ethyl applied at its field‐recommended rate was collected from a wheat field in Huaiyuan County, Anhui Province, China. This study aimed to determine the resistance profile of AHHY to acetyl‐CoA carboxylase (ACCase) inhibitors and to investigate its mechanisms of resistance to fenoxaprop. RESULTS: Single‐dose testing indicated that the AHHY population had evolved resistance to fenoxaprop. The partial carboxyltransferase domain of ACCase in P. fugax was amplified and compared. Four loci encoding plastidic ACCase were isolated from both the resistant and sensitive individuals. Combining gene sequencing with the derived cleaved amplified polymorphic sequence assay, we found that 100% of the plants of AHHY carried Trp‐1999‐Ser mutation in their ACCase1, 1–2 allele. Whole‐plant dose–response bioassay indicated that AHHY was highly resistant to fenoxaprop and pinoxaden (resistance index (RI) ≥ 10) with low resistance to clodinafop‐propargyl, sethoxydim, and clethodim (2 ≤ RI < 5). Pre‐treatment with piperonyl butoxide largely reduced (55%) the weed's resistance to fenoxaprop. Both basal and fenoxaprop‐induced glutathione S ‐transferases activities toward 1‐chloro‐2, 4‐dinitrobenzene were significantly higher in resistant plants than in susceptible plants. CONCLUSION: This study revealed that P. fugax had multiple alleles encoding plastidic ACCase, and reported for the first time the occurrence of Trp‐1999‐Ser mutation and non‐target‐site resistance in this species. Fenoxaprop resistance in AHHY plants was conferred by target‐site mutation and P450s‐involved enhanced metabolism. © 2019 Society of Chemical Industry Abstract : This study revealed that Polypogon fugax has multiple alleles encoding plastidic ACCase. Fenoxaprop‐ P ‐ethyl resistance in AHHY population was conferred by ACCase1, 1–2 allele Trp‐1999‐Ser mutation and P450s‐involved enhanced metabolism.