Analysis of thrombin‐antithrombin complex formation using microchip electrophoresis and mass spectrometry. Issue 21 (13th August 2019)

• Record Type: Journal Article
• Authors: Nielsen, Jacob B.; Nielsen, Anna V.; Carson, Richard H.; Lin, Hsien‐Jung L.; Hanson, Robert L.; Sonker, Mukul; Mortensen, Daniel N.; Price, John C.; Woolley, Adam T.
• Is Part Of: Electrophoresis. Volume 40:Issue 21(2019)
• Page Start: 2853
• Page End: 2859
• Publication Date: 2019-08-13
• Subjects: Biomarkers -- Disease diagnosis -- Dot blot -- Point‐of‐care diagnostics -- Protein surface modification; Electrophoresis -- Periodicals; Electrophoresis -- Periodicals; 541.372
• DOI: 10.1002/elps.201900235
• Languages: English
• ISSNs: 0173-0835

Abstract

Abstract: Preterm birth (PTB) related health problems take over one million lives each year, and currently, no clinical analysis is available to determine if a fetus is at risk for PTB. Here, we describe the preparation of a key PTB risk biomarker, thrombin‐antithrombin (TAT), and characterize it using dot blots, MS, and microchip electrophoresis (µCE). The pH for fluorescently labeling TAT was also optimized using spectrofluorometry and spectrophotometry. The LOD of TAT was measured in µCE. Lastly, TAT was combined with six other PTB risk biomarkers and separated in µCE. The ability to make and characterize TAT is an important step toward the development of an integrated microfluidic diagnostic for PTB risk.