High levels of ANXA2 are characteristic of malignant salivary gland tumors. (5th August 2019)

• Record Type: Journal Article
• Authors: Cardoso, Claudio Marcelo; de Jesus, Sabrina Ferreira; de Souza, Marcela Gonçalves; Queiroz, Lorena dos Reis Pereira; Santos, Eloa Mangabeira; dos Santos, Erivelton Pereira; Oliveira, Luis Paulo; Cordeiro Santos, Cristiane Karine; Santos, Sérgio Henrique Sousa; de Paula, Alfredo Maurício Batista; Farias, Lucyana Conceição; Guimaraes, Andre Luiz Sena
• Is Part Of: Journal of oral pathology & medicine. Volume 48:Number 10(2019)
• Page Start: 929
• Page End: 934
• Publication Date: 2019-08-05
• Subjects: gland -- malignancy -- metastasis -- neoplasm -- oral cancer; Dentistry -- Periodicals; Teeth -- Diseases -- Periodicals; 617
• DOI: 10.1111/jop.12932
• Languages: English
• ISSNs: 0904-2512

Abstract

Abstract: Objective: Malignant salivary gland tumors (MSGTs) present different phenotypic characteristics and various clinical outcomes, which proved to be a diagnostic challenge. Considering the heterogeneity of MSGT, this study aims to identify molecule related to the nature of MSGT. Methods: For screening, proteomic analysis comparing MSGT with pleomorphic adenoma (PA) and salivary gland was performed. The MSGT‐associated protein which presented in the higher number in the Gene Expression Omnibus (GEO) database was selected. To validate the data, immunohistochemistry (IHC) was performed in 14 patients with PA, 22 patients with MSGT, and 14 controls. Results: 16 proteins were associated with MSGT. ANXA2 was the primary protein, according to GEO database analyses. ANXA2 was most expressed in the cell membrane. However, some ANXA2 staining was also observed in the cytoplasm and nucleus. ANXA2 was highly expressed in MSGT in comparison with control. Also, ANXA2 has a higher expression in adenocarcinoma not otherwise specified (ANOS) and myoepithelial carcinoma (MC) in comparison with PA. Conclusion: In conclusion, this study demonstrated that MSGT presented higher levels of ANXA2 in comparison with normal salivary glands. Also, ANXA2 might be interesting as a molecular marker of ANOS and MS.