Catalyst-proximity protein chemical labelling on affinity beads targeting endogenous lectins. Issue 88 (18th October 2019)

• Record Type: Journal Article
• Authors: Tsushima, Michihiko; Sato, Shinichi; Niwa, Tatsuya; Taguchi, Hideki; Nakamura, Hiroyuki
• Is Part Of: Chemical communications. Volume 55:Issue 88(2019)
• Page Start: 13275
• Page End: 13278
• Publication Date: 2019-10-18
• Subjects: Chemistry -- Periodicals; 540
• DOI: 10.1039/c9cc05231c
• Languages: English
• ISSNs: 1359-7345

Abstract

Abstract : Catalyst-proximity labelling on affinity beads enables the identification of ligand-binding proteins such as lectins, which cannot be analyzed by conventional techniques. 1-Methyl-4-arylurazole (MAUra) efficiently labels proteins bound to the beads. Abstract : Magnetic affinity beads functionalized with lactose and ruthenium/dcbpy complexes were developed. Using MAUra, a catalyst-proximity labelling reagent, the catalytic labeling of lactose-binding proteins was achieved with high selectivity on the beads. The first unbiased identification of cellular endogenous lectins bound to lactose (galectin-1 and galectin-3) was achieved with chemical labelling on the affinity beads.