Transcriptomic responses of Artemia salina exposed to an environmentally relevant dose of Alexandrium minutum cells or Gonyautoxin2/3. (January 2020)

• Record Type: Journal Article
• Authors: Yi, Xianliang; Zhang, Keke; Liu, Renyan; Giesy, John P.; Li, Zhaochuan; Li, Wentao; Zhan, Jingjing; Liu, Lifen; Gong, Yufeng
• Is Part Of: Chemosphere. Volume 238(2020)
• Publication Date: 2020-01
• Subjects: Hazardous algal bloom -- Gonyautoxins -- Transcriptomics -- Acute test -- Microalgae -- Phytoplankton; Pollution -- Periodicals; Pollution -- Physiological effect -- Periodicals; Environmental sciences -- Periodicals; Atmospheric chemistry -- Periodicals; 551.511
• DOI: 10.1016/j.chemosphere.2019.124661
• Languages: English
• ISSNs: 0045-6535

Abstract

Abstract: Toxicities of the marine algae Alexandrium minutum and its excreted gonyautoxins (GTXs) to the marine crustacean Artemia salina were investigated. Mortality was observed for neither larvae nor adult A. salina exposed to A. minutum at a density of 5000 cells/mL or 0.5 μM GTX2/3. After exposure, the full transcriptome of adult A. salina was assembled and functionally annotated. A total of 599, 286 transcripts were obtained, which were clustered into 515, 196 unigenes. Results of the transcriptional effect level index revealed that direct exposure to the toxic algae A. minutum caused greater alterations in the transcriptome than did exposure to the extracellular product GTX2/3. Mechanisms of effects were different between exposure of A. salina to A. minutum cells or GTX2/3. Exposure to A. minutum modulated formation of the ribonucleoprotein complex and metabolism of amino acids and lipids in A. salina . Exposure to GTX2/3 exposure inhibited expression of genes related to metabolism of chitin, which might result in disruption of molting process or disturbed sheath morphogenesis. Overall, effects on transcription observed in this study represent the first report based on application of next generation sequencing techniques to investigate the transcriptomic response of A. salina exposed to an environmentally realistic level of A. minutum or GTX2/3. Graphical abstract: Image 1 Highlights: A. salina was not very sensitive to A. minutum or GTX2/3 exposure. Exposure to A. minutum or GTX2/3 induced HSP70 expression in A. salina . Transcriptional responses of A. salina to A. minutum or GTX2/3 were different. Induced ribonucleoprotein expression protected A. salina from exposure to A. minutum . Exposure to GTX2/3 inhibited formation of chitin in A. salina .