A self-enhanced renewable electrochemiluminescence biosensing platform for ultrasensitive detection of sialic acid. (5th December 2019)

• Record Type: Journal Article
• Authors: Fang, Dandan; Zhang, Shupei; Dai, Hong; Hong, Zhensheng; Lin, Yanyu
• Is Part Of: Electrochimica acta. Volume 326(2019)
• Publication Date: 2019-12-05
• Subjects: Renewable electrochemiluminescence -- Self-cleaning -- Self-enhanced -- 4-Mercaptophenylboronic acid -- Nss-TiO2-RM -- Sialic acids; Electrochemistry -- Periodicals; Electrochemistry, Industrial -- Periodicals; 541.37
• DOI: 10.1016/j.electacta.2019.134956
• Languages: English
• ISSNs: 0013-4686

Abstract

Abstract: Herein, a boronate-affinity renewable electrochemiluminescence (ECL) biosensor was constructed based on a highly efficient Ru (II)-based composite for the sensitive detection of sialic acid (SA). Specifically, the Bi nanobelts (Bi NBs) was first used as coreactant to prepare a self-enhanced Ru(bpy)3 2+ -Bi NBs composite, producing a strong ECL signal by shortening electronic transmission distance and reducing the energy loss. Additionally, HNO3 -assisted synthesized rutile TiO2 mesocrystals (Nss-TiO2 -RM) with nanoporous nature and single-crystal like structure was employed as scaffold, increasing the immobilization amount of Ru(bpy)3 2+ and Bi NBs, further enhancing ECL signal. Promisingly, 4-Mercaptophenylboronic acid (4-MPBA) as bionic recognition unit could substitute antibody to capture SA, and moreover a self-cleaning electrode could be obtained after treated by 0.1 M PBS (pH 6). Under optimal conditions, a sandwich-like renewable ECL biosensor was constructed for sensitive detection of SA in the range of 3.5 × 10 −5 to 350 ng/mL with a low detection limit of 1.17 × 10 −5 ng/mL. This work provided a new thought for other biomarkers determination.