A perylenediimide based 'on-off' chemosensor for the detection of nucleoside triphosphates: an efficient ensemble for monitoring alkaline phosphatase activity. Issue 41 (9th October 2019)

• Record Type: Journal Article
• Authors: Mittal, Lalit Singh; Sharma, Poonam; Kaur, Navdeep; Singh, Prabhpreet
• Is Part Of: Analytical methods. Volume 11:Issue 41(2019)
• Page Start: 5320
• Page End: 5327
• Publication Date: 2019-10-09
• Subjects: Chemistry, Analytic -- Periodicals; Analytical biochemistry -- Periodicals; Chemical laboratories -- Standards -- Periodicals; 543.1905
• DOI: 10.1039/c9ay01608b
• Languages: English
• ISSNs: 1759-9660

Abstract

Abstract : Cationic perylenediimide (BAB-PDI ) showed fluorescence sensing toward nucleoside polyphosphates (GTP, LOD = 19.3 nM) in an aqueous medium. A BAB-PDI + GTP ensemble could be used to monitor the activity of alkaline phosphatase enzyme. Abstract : The evaluation of the optical properties of BAB-PDI towards nucleoside polyphosphates (NPPs) in 90% aqueous media (pH 7.2) revealed that upon addition of guanosine triphosphate (GTP), the fluorescence intensity of BAB-PDI was >85% quenched. In the case of adenosine triphosphate (ATP), uridine triphosphate (UTP) and cytidine triphosphate (CTP), only 30–65% quenching of fluorescence intensity was observed. Other NPPs such as nucleoside diphosphates and monophosphates, respectively, caused 20–30% and <5% quenching of fluorescence intensity of BAB-PDI due to fewer negative charges on di- and mono-phosphates. The minimum limit of detection of GTP reached 19.3 nM. The application of BAB-PDI in monitoring enzyme (alkaline phosphatase) activity in aqueous solution using a BAB-PDI + GTP ensemble has also been demonstrated.