Glaciihabitans arcticus sp. nov., a psychrotolerant bacterium isolated from Arctic soil. Issue 8 (18th June 2019)

• Record Type: Journal Article
• Authors: Dahal, Ram Hari; Kim, Jaisoo
• Is Part Of: International journal of systematic and evolutionary microbiology. Volume 69:Issue 8(2019)
• Page Start: 2492
• Page End: 2497
• Publication Date: 2019-06-18
• Subjects: Glaciihabitans arcticus sp. nov. -- Arctic soil -- psychrotolerant -- DAB; Microbiology -- Periodicals; Microbiology -- Classification -- Periodicals; 579
• DOI: 10.1099/ijsem.0.003520
• Languages: English
• ISSNs: 1466-5026

Abstract

Abstract : A non-motile, Gram-stain-positive, rod-shaped bacterium, designated RP-3-7 T, was isolated from soil sampled at the Arctic region in Cambridge Bay, NU, Canada. Cells were strictly aerobic, non-spore-forming, catalase-positive and oxidase-positive. Colonies on Reasoner's 2A agar plates were pale yellow-coloured. Strain RP-3-7 T was psychrotolerant and grew optimally at 15–20 °C. Strain RP-3-7 T assimilated d -glucose, d -mannitol, l -arabinose and l -proline; tolerated only 0.5 % NaCl (w/v). Phylogenetic analysis based on its 16S rRNA gene sequence revealed that strain RP-3-7 T formed a lineage within the family Microbacteriaceae and clustered with members of the genus Glaciihabitans . The closest member was Glaciihabitans tibetensis MP203 T (98.26 % sequence similarity). The major respiratory quinone was MK-10. The major polar lipids were diphosphatidylglycerol, phosphatidylglycerol and an unidentified glycolipid. The major cellular fatty acids were anteiso-C15 : 0, iso-C16 : 0, anteiso-C17 : 0 and anteiso-C15 : 1 A. The diagnostic amino acid was 2, 4-diaminobutyric acid and whole-cell sugars were xylose, rhamnose and mannose. The DNA G+C content of strain RP-3-7 T was 66.9 mol%. The average nucleotide identity and in silico DNA–DNA relatedness values between strain RP-3-7 T and Glaciihabitans tibetensis MP203 T were 73.79 and 20.1 %, respectively. Based on the polyphasic, genomic and phylogenetic data, strain RP-3-7 T represents a novel species of the genus Glaciihabitans, for which the name Glaciihabitans arcticus sp. nov. is proposed. The type strain is RP-3-7 T (=KEMB 9005-731 T =KACC 21151 T =NBRC 113769 T ).