Hymenobacter metallilatus sp. nov., isolated from abandoned lead–zinc ore. Issue 7 (23rd May 2019)

• Record Type: Journal Article
• Authors: Feng, Guang-Da; Zhang, Jun; Zhang, Xian-Jiao; Wang, Sheng-Nan; Xiong, Xiong; Zhang, Yu-Lian; Huang, Hua-Rui; Zhu, Hong-Hui
• Is Part Of: International journal of systematic and evolutionary microbiology. Volume 69:Issue 7(2019)
• Page Start: 2142
• Page End: 2146
• Publication Date: 2019-05-23
• Subjects: Hymenobacter metallilatus -- ANI -- dDDH -- plasmid -- whole genome sequence; Microbiology -- Periodicals; Microbiology -- Classification -- Periodicals; 579
• DOI: 10.1099/ijsem.0.003450
• Languages: English
• ISSNs: 1466-5026

Abstract

Abstract : An aerobic, non-motile, Gram-stain-negative, red-to-pinkish and rod-shaped bacterium, designated 9PBR-2 T, was isolated from an abandoned lead–zinc ore sample collected from Meizhou, Guangdong Province, PR China. Phylogenetic analyses based on 16S rRNA gene sequences showed that strain 9PBR-2 T belongs to the genus Hymenobacte r and was most closely related to Hymenobacter rigui KCTC 12533 T (98.0 %), Hymenobacter swuensis KCTC 32018 T (97.8 %) and Hymenobacter perfusus LMG 26000 T (97.6 %). The calculated average nucleotide identity values based on whole genome sequences between strain 9PBR-2 T and closely related type strains ranged from 81.3 to 84.1 %. Correspondingly, the digital DNA-DNA hybridization values ranged from 25.5 to 28.1 %. The major fatty acids of strain 9PBR-2 T were iso-C15:0, anteiso-C15:0, C16:1 ω 5 c, summed feature 3 (C16:1 ω 6 c and/or C16:1 ω 7 c ) and summed feature 4 (iso-C17:1 I and/or anteiso-C17:1 B). It contained menaquinone 7 (MK-7) as the major isoprenoid quinone and phosphatidylethanolamine as the major polar lipid. The genomic DNA G+C content based on whole genome sequence was 59.8 mol%. Characterization based on phylogenetic, chemotaxonomic and phenotypic analyses clearly indicated that strain 9PBR-2 T represents a novel species of the genus Hymenobacter, for which the name Hymenobacter metallilatus sp. nov. is proposed. The type strain is 9PBR-2 T (=GDMCC 1.1492 T =JCM 32699 T ).