Zinc oxide and copper nanoparticles addition in universal adhesive systems improve interface stability on caries-affected dentin. (December 2019)

• Record Type: Journal Article
• Authors: Gutiérrez, Mario Felipe; Bermudez, Jorge; Dávila-Sánchez, Andrés; Alegría-Acevedo, Luisa F.; Méndez-Bauer, Luján; Hernández, Marcela; Astorga, Jessica; Reis, Alessandra; Loguercio, Alessandro D.; Farago, Paulo V.; Fernández, Eduardo
• Is Part Of: Journal of the mechanical behavior of biomedical materials. Volume 100(2019)
• Publication Date: 2019-12
• Subjects: Universal adhesive system -- Zinc oxide -- Copper -- Nanoparticles -- Microtensile bond strength -- Nanoleakage; Biomedical materials -- Periodicals; Biomedical materials -- Mechanical properties -- Periodicals; Biomedical materials; Biomedical materials -- Mechanical properties; Periodicals; Electronic journals; 610.28
• DOI: 10.1016/j.jmbbm.2019.07.024
• Languages: English
• ISSNs: 1751-6161

Abstract

Abstract: This study evaluated the MMP inhibition of the zinc oxide and copper nanoparticles (ZnO/CuNp), and the effects of their addition into adhesives on antimicrobial activity (AMA), ultimate tensile strength (UTS), in vitro degree of conversion ( in vitro -DC), as well as, resin–dentin bond strength (μTBS), nanoleakage (NL) and in situ -DC on caries-affected dentin. Anti-MMP activity was evaluated for several MMPs. ZnO/CuNp (0% [control]; 5/0.1 and 5/0.2 wt%) were added into Prime&Bond Active (PBA) and Ambar Universal (AMB). The AMA was evaluated against Streptococcus mutans . UTS were tested after 24 h and 28d. After induced caries, adhesives and composite were applied to flat dentin surfaces, and specimens were sectioned to obtain resin–dentin sticks. μTBS, NL, in vitro -DC and in situ -DC were evaluated after 24 h. ANOVA and Tukey's test were applied (α = 0.05). ZnO/CuNp demonstrated anti-MMP activity ( p < 0.05). The addition of ZnO/CuNp increased AMA and UTS (AMB; p < 0.05). UTS for PBA, in vitro -DC, in situ -DC and μTBS for both adhesives were maintained with ZnO/CuNp ( p > 0.05). However, lower NL was observed for ZnO/CuNp groups ( p < 0.05). The addition of ZnO/CuNp in adhesives may be an alternative to provide antimicrobial, anti-MMP activities and improves the integrity of the hybrid layer on caries-affected dentin. Highlights: Zinc oxide and copper nanoparticles were added in different concentrations to two universal adhesive systems. Addition of zinc oxide and copper nanoparticles did not affect several mechanical and biological properties tested. Zinc oxide and copper nanoparticles improve stability of adhesive–dentin interfaces. Addition of zinc oxide and copper nanoparticles significant increase the antimicrobial activity against S. mutans.