Emulating endothelial dysfunction by implementing an early atherosclerotic microenvironment within a microfluidic chip. Issue 21 (30th September 2019)

• Record Type: Journal Article
• Authors: Shin, Yujin; Lim, Seongjin; Kim, Jinwon; Jeon, Jessie S.; Yoo, Hongki; Gweon, Bomi
• Is Part Of: Lab on a chip. Volume 19:Issue 21(2019)
• Page Start: 3664
• Page End: 3677
• Publication Date: 2019-09-30
• Subjects: Miniature electronic equipment -- Periodicals; Combinatorial chemistry -- Periodicals; Biotechnology -- Periodicals; 543.0813
• DOI: 10.1039/c9lc00352e
• Languages: English
• ISSNs: 1473-0197

Abstract

Abstract : The pathophysiological phenotype and function of endothelial cells (ECs) in atherosclerotic lesions are replicated within a micro-channel by mimicking the microenvironment of the lesions. Abstract : Recent studies on endothelial dysfunction in relation to vascular diseases including atherosclerosis have highlighted the key contribution of the microenvironment of endothelial cells (ECs). By mimicking the microenvironment of early atherosclerotic lesions, here, we replicate the pathophysiological phenotype and function of ECs within microchannels. Considering the elevated deposition of fibronectin (FN) in early atherosclerotic plaques and the close correlation between the vascular stiffness and the progression of atherosclerosis, we utilized FN coated hydrogels with increased stiffness for endothelial substrates within the microchannels. As a result, we demonstrated that endothelial integrity on FN coated microchannels is likely to be undermined exhibiting a random orientation in response to the applied fluid flow, notable disruption of vascular endothelial cadherins (VE-cadherins), and higher endothelial permeability as opposed to that on microchannels coated with collagen (CL), the atheroresistant vascular model.