Styrene maleic acid copolymer induces pores in biomembranes. Issue 39 (20th September 2019)

• Record Type: Journal Article
• Authors: Orwick Rydmark, Marcella; Christensen, Mikkel Killingmoe; Köksal, Elif Senem; Kantarci, Ilayda; Kustanovich, Kiryl; Yantchev, Ventsislav; Jesorka, Aldo; Gözen, Irep
• Is Part Of: Soft matter. Volume 15:Issue 39(2019)
• Page Start: 7934
• Page End: 7944
• Publication Date: 2019-09-20
• Subjects: Soft condensed matter -- Periodicals; 530.413
• DOI: 10.1039/c9sm01407a
• Languages: English
• ISSNs: 1744-683X

Abstract

Abstract : The experimental observations revealed that the interaction of styrene maleic acid with lipid membranes results in formation of toroidal pores. Abstract : We investigated the interactions between styrene–maleic acid (SMA) copolymers and phospholipid bilayers, using confocal microscopy and surface acoustic wave resonance (SAR) sensing. For the first time we experimentally observed and followed pore formation by SMA copolymers in intact supported bilayers and unilamellar vesicles, showing that fluorescein, a water-soluble organic compound with a mean diameter of 6.9 Å, can traverse the membrane. Our findings are in agreement with recent theoretical predictions, which suggested that SMA copolymers may insert along the plane of the bilayer to form stable toroidal pores.