Polymer brush based fluorescent immunosensor for direct monitoring of interleukin-1β in rat blood. Issue 19 (16th August 2019)

• Record Type: Journal Article
• Authors: Deng, Fei; Li, Yi; Hossain, Md Jakir; Kendig, Michael D.; Arnold, Ria; Goldys, Ewa M.; Morris, Margaret J.; Liu, Guozhen
• Is Part Of: Analyst. Volume 144:Issue 19(2019)
• Page Start: 5682
• Page End: 5690
• Publication Date: 2019-08-16
• Subjects: Chemistry, Analytic -- Periodicals; 543
• DOI: 10.1039/c9an01300h
• Languages: English
• ISSNs: 0003-2654

Abstract

Abstract : A sandwich immunosensor was successfully developed for monitoring of interleukin-1β (IL-1β) in rat whole blood. Abstract : A sandwich immunosensor was successfully developed for monitoring of interleukin-1β (IL-1β) in rat whole blood. The substrate stainless steel (SS) was first coated with a polydopamine layer and subsequently grafted with poly(ethylene glycol) methacrylate brushes, onto which a sandwich immunosensor was modified for detection of IL-1β. The device has been successfully applied for monitoring of IL-1β with a limit of detection of 4.7 pg mL −1, and a linear detection range of 12.5–200 pg mL −1 . Good specificity and selectivity for monitoring of IL-1β in rat macrophage secretion were achieved. Furthermore, this device was validated by detection of IL-1β in rat whole blood samples with greater concentrations observed in obese rats compared to control, and strong positive correlation between concentrations of IL-1β and blood glucose. These results suggest this device is feasible for direct detection of target analytes in biological samples.