Establishment of a Rat Model of Superior Sagittal-Sinus Occlusion and Recanalization via a Thread-Embolism Method. (15th September 2019)

• Record Type: Journal Article
• Authors: Wang, Wei; Mu, Shuwen; Xu, Weiming; Liang, Shengxiang; Lin, Ruhui; Li, Zuanfang; Liu, Qinghong; Yi, Gaocheng; Xue, Liang; Wang, Shousen
• Is Part Of: Neuroscience. Volume 416(2019)
• Page Start: 41
• Page End: 49
• Publication Date: 2019-09-15
• Subjects: ADC apparent diffusion coefficient -- BBB blood–brain barrier -- CBF cerebral blood flow -- CVST cerebral venous sinus thrombosis -- DWI diffusion-weighted imaging -- ELISA Enzyme-linked immunosorbent assay -- HE hematoxylin and eosin -- LSCI laser speckle contrast imaging -- MRA magnetic resonance angiography -- MRI magnetic resonance imaging -- ROI region of interest -- SSS superior sagittal sinus -- TJ tight junction -- ZO zonula occluden; animal model -- CVST -- recanalization -- collateral circulation -- brain edema; Neurochemistry -- Periodicals; Neurophysiology -- Periodicals; Neurology -- Periodicals; Neurochimie -- Périodiques; Neurophysiologie -- Périodiques; Neurochemistry; Neurophysiology; Electronic journals; Periodicals; Electronic journals; 612.8
• DOI: 10.1016/j.neuroscience.2019.07.044
• Languages: English
• ISSNs: 0306-4522

Abstract

Abstract: There are many uncontrollable factors in the pathogenesis of cerebral venous sinus thrombosis (CVST). In order to further explore the pathophysiology and morphology of CVST, it is necessary to establish a highly compatible CVST animal model that can standardize the site and stage of venous thrombosis. The present study employed the insertion of a self-made thread embolism into the superior sagittal sinus (SSS) to establish a rat model of SSS occlusion that emulates CVST. The thread embolism was removed after 6 h of SSS occlusion in order to achieve recanalization. After successful preparation of the model, the cerebral blood flow (CBF) status and ultrastructural changes of the blood–brain barrier (BBB) were monitored. This CVST model was able to achieve continuously high occlusion of SSS. The parasagittal venous-collateral circulation underwent extensive compensation and recombination, which alleviated blood flow stasis and brain tissue hypoxia caused by restricted reflux. Removing SSS occlusion significantly improved cerebral circulation, reduced brain edema, and accelerated the receding of brain edema. This study established a new model of acute occlusion and recanalization of SSS in rats via a thread-embolism method, which standardized the ischemic site and stage of venous thrombosis. In addition, our study suggests that promoting collateral circulation may be a potential treatment for promoting brain protection. Highlights: A model of acute occlusion and recanalization of the SSS has been established. Brain edema after SSS occlusion is slightly reversible. Compensatory effect of venous collateral circulation can improve brain edema. Early removal of the SSS occlusion can improve cerebral blood circulation.