Marinomonas piezotolerans sp. nov., isolated from deep-sea sediment of the Yap Trench, Pacific Ocean. Issue 3 (16th January 2019)

• Record Type: Journal Article
• Authors: Yu, Libo; Zhou, Zhidong; Wei, Shiping; Xu, Xiashutong; Wang, Qilin; Xu, Guangxin; Tang, Xixiang; Yang, Qian
• Is Part Of: International journal of systematic and evolutionary microbiology. Volume 69:Issue 3(2019)
• Page Start: 739
• Page End: 744
• Publication Date: 2019-01-16
• Subjects: Marinomonas piezotolerans sp. nov. -- deep-sea sediment -- Yap Trench; Microbiology -- Periodicals; Microbiology -- Classification -- Periodicals; 579
• DOI: 10.1099/ijsem.0.003227
• Languages: English
• ISSNs: 1466-5026

Abstract

Abstract : A Gram-stain-negative, aerobic and rod-shaped strain, YLB-05 T, was isolated from a sample of deep-sea sediment (depth, 6796 m) from the Yap Trench. It was motile, oxidase-positive and catalase-positive. Growth was observed at salinities of 1–12 % (NaCl, w/v), with the optimum at 5 %. The strain was able to thrive at low (4 °C) temperatures, with the optimum at 37 °C, but did not grow at 50 °C. The optimum pressure for growth was 0.1 MPa with tolerance up to 50 MPa. The 16S rRNA gene sequencing analysis showed that YLB-05 T was most closely related to Marinomonas communis LMG 2864 T (97.8 %). Phylogenetic analysis placed strain YLB-05 T within the genus Marinomonas . The average nucleotide identity and the DNA–DNA hybridization values between strain YLB-05 T and closely related type strains were below the respective thresholds for species differentiation. The principal fatty acids were C16 : 0, summed feature 8 (C18 : 1 ω 7 c and/or C18 : 1 ω 6 c ) and summed feature 3 (C16 : 1 ω 7 c/C16 : 1 ω 6 c ). The DNA G+C content was 45.7 mol%. The respiratory quinone was determined to be Q-8. The polar lipids were an unidentified phospholipid and an unidentified aminophospholipid. The combined genotypic and phenotypic data showed that strain YLB-05 T represents a novel species within the genus Marinomonas, for which the name Marinomonas piezotolerans sp. nov. is proposed, with the type strain YLB-05 T (=MCCC 1A12712 T =KCTC 62812 T ).