Micro Array-Assisted Analysis of Anti-Schistosome Glycan Antibodies Elicited by Protective Vaccination With Irradiated Cercariae. (18th December 2018)

• Record Type: Journal Article
• Authors: Yang, Y Y Michelle; Wilson, R Alan; Thomas, Steffan R L; Kariuki, Thomas M; van Diepen, Angela; Hokke, Cornelis H
• Is Part Of: Journal of infectious diseases. Volume 219:Number 10(2019)
• Page Start: 1671
• Page End: 1680
• Publication Date: 2018-12-18
• Subjects: anti-glycan response -- schistosomiasis -- vaccination -- irradiated cercariae -- glycan -- antibodies -- baboon vaccination -- glycan-microarray -- antibodies -- schistosomula; Communicable diseases -- Periodicals; Diseases -- Causes and theories of causation -- Periodicals; Medicine -- Periodicals; Communicable Diseases -- Periodicals; Electronic journals; 616.9
• DOI: 10.1093/infdis/jiy714
• Languages: English
• ISSNs: 0022-1899

Abstract

Abstract : Baboons vaccinated with radiation-attenuated schistosome cercariae are resistant against infection. In vaccinated animals, serum IgG, but not IgM, against multi-fucosylated schistosome glycans are boosted upon subsequent immunisations and sustained during challenge, suggesting a possible role for these antibodies in protection. Abstract: Baboons vaccinated with radiation-attenuated cercariae develop high levels of protection against schistosome infection, correlating to high antibody titres towards schistosome antigens with unknown molecular identity. Using a microarray consisting of glycans isolated from different life-stages of schistosomes, we studied the anti-glycan immunoglobulin (Ig) G and IgM responses in vaccinated and challenged baboons over a time course of 25 weeks. Anti-glycan IgM responses developed early after vaccination, but did not rise in response to later vaccinations. In contrast, anti-glycan IgG developed more slowly, but was boosted by all five subsequent vaccinations. High IgM and IgG levels against O-glycans and glycosphingolipid glycans of cercariae were observed. At the time of challenge, while most antibody levels decreased in the absence of vaccination, IgG towards a subset of glycans containing multiple-fucosylated motifs remained high until 6 weeks post-challenge during challenge parasite elimination, suggesting a possible role of this IgG in protection.