Extracellular vesicles reveal abnormalities in neuronal iron metabolism in restless legs syndrome. Issue 7 (21st March 2019)

• Record Type: Journal Article
• Authors: Chawla, Sahil; Gulyani, Seema; Allen, Richard P; Earley, Christopher J; Li, Xu; Van Zijl, Peter; Kapogiannis, Dimitrios
• Is Part Of: Sleep. Volume 42:Issue 7(2019)
• Publication Date: 2019-03-21
• Subjects: restless legs syndrome -- exosomes -- extracellular vesicles -- iron -- ferritin; Sleep -- Physiological aspects -- Periodicals; Sleep disorders -- Periodicals; Sommeil -- Aspect physiologique -- Périodiques; Sommeil, Troubles du -- Périodiques; Sleep disorders; Sleep -- Physiological aspects; Sleep -- physiological aspects; Sleep Wake Disorders; Psychophysiology; Electronic journals; Periodicals; 616.8498
• DOI: 10.1093/sleep/zsz079
• Languages: English
• ISSNs: 0161-8105

Abstract

Abstract: Study Objectives: Determine abnormalities in levels of iron-management proteins in neuronal origin-enriched extracellular vesicles (nEVs) in restless legs syndrome (RLS). Methods: We used immunoprecipitation for neuronal marker L1CAM to isolate nEVs from the serum of 20 participants with RLS from a study including magnetic resonance imaging (MRI) determinations of iron deposition in the substantia nigra and hematologic parameters and 28 age- and sex-matched Controls. Results: RLS compared with Control participants showed higher levels of nEV total ferritin but similar levels of transferrin receptor and ferroportin. Western blot analysis showed that heavy- but not light-chain ferritin was increased in nEVs of RLS compared with Control participants. In RLS but not Control participants, nEV total ferritin was positively correlated with systemic iron parameters; the two groups also differed in the relation of nEV total ferritin to MRI measures of iron deposition in substantia nigra. Conclusions: Given the neuronal origin and diversity of EV cargo, nEVs provide an important platform for exploring the underlying pathophysiology and possible biomarkers of RLS.