A Moso Bamboo Drought-Induced 19 Protein, PeDi19-4, Enhanced Drought and Salt Tolerance in Plants via the ABA-Dependent Signaling Pathway. (19th November 2018)

• Record Type: Journal Article
• Authors: Wu, Min; Cai, Ronghao; Liu, Huanlong; Li, Fei; Zhao, Yang; Xiang, Yan
• Is Part Of: Plant & cell physiology. Volume 60:Number 7(2019)
• Page Start: e1
• Page End: e14
• Publication Date: 2018-11-19
• Subjects: ABA -- Drought and salt stresses -- Moso bamboo -- PeDi19-4 -- Target genes -- Transcription activator; Plant physiology -- Periodicals; Microbiology -- Periodicals; Cytology -- Periodicals; Cell Physiology -- Periodicals; Plant Physiological Phenomena -- Periodicals; Cytology; Microbiology; Plant physiology; Periodicals; 571.205
• DOI: 10.1093/pcp/pcy196
• Languages: English
• ISSNs: 0032-0781

Abstract

Abstract: Here, 10 drought-induced 19 (Di19) proteins from Phyllostachys edulis were analyzed and an important stress-related candidate gene ( PeDi19-4 ) was isolated based on analysis of phylogenetic relationships and expression profiles. PeDi19-4 is a nuclear localization protein that can bind the conserved TACA(A/G)T sequence, as determined using enzyme-linked immunosorbent assay (EMSA). PeDi19-4 has no transcriptional activity in yeast but functions as a transcription activator in plants. Overexpression of PeDi19-4 in rice and Arabidopsis thaliana enhanced drought and salt tolerance as determined through phenotypic analysis and the use of stress-associated physiological indicators. PeDi19-4 transgenic plants showed increased sensitivity to ABA during seed germination and early seedling growth. Additionally, transgenic rice accumulated more ABA than wild-type plants under drought and salt stress conditions. Moreover, the stomata of PeDi19-4 -overexpressing plants changed significantly with ABA treatment. RNA sequencing revealed that PeDi19-4 regulated the expression of a wide spectrum of stress-/ABA-responsive differentially expressed genes. The stress-responsive genes ( OsZFP252 and OsNAC6 ) and ABA-responsive genes ( OsBZ8 and OsbZIP23 ) were direct targets of PeDi19-4 . Our research indicated that PeDi19-4 enhanced drought and salt tolerance in plants via the ABA-dependent signaling pathway.