Disruption of the Gene trx-m1 Impedes the Growth of Anabaena sp. PCC 7120 under Nitrogen Starvation. (8th April 2019)

• Record Type: Journal Article
• Authors: Deschoenmaeker, Fr�d�ric; Mihara, Shoko; Niwa, Tatsuya; Taguchi, Hideki; Nomata, Jiro; Wakabayashi, Ken-Ichi; Hisabori, Toru
• Is Part Of: Plant & cell physiology. Volume 60:Number 7(2019)
• Page Start: 1504
• Page End: 1513
• Publication Date: 2019-04-08
• Subjects: Anabaena -- Heterocyst -- Nitrogen starvation -- Redox regulation -- Thioredoxin; Plant physiology -- Periodicals; Microbiology -- Periodicals; Cytology -- Periodicals; Cell Physiology -- Periodicals; Plant Physiological Phenomena -- Periodicals; Cytology; Microbiology; Plant physiology; Periodicals; 571.205
• DOI: 10.1093/pcp/pcz056
• Languages: English
• ISSNs: 0032-0781

Abstract

Abstract: Cyanobacteria possess a sophisticated photosynthesis-based metabolism with admirable plasticity. This plasticity is possible via the deep regulation network, the thiol-redox regulations operated by thioredoxin (hereafter, Trx). In this context, we characterized the Trx- m 1-deficient mutant strain of Anabaena sp., PCC 7120 (shortly named A .7120), cultivated under nitrogen limitation. Trx- m 1 appears to coordinate the nitrogen response and its absence induces large changes in the proteome. Our data clearly indicate that Trx- m 1 is crucial for the diazotrophic growth of A .7120. The lack of Trx- m 1 resulted in a large differentiation of heterocysts (>20% of total cells), which were barely functional probably due to a weak expression of nitrogenase. In addition, heterocysts of the mutant strain did not display the usual cellular structure of nitrogen-fixative cells. This unveiled why the mutant strain was not able to grow under nitrogen starvation.