Analysis of an improved Cyanophora paradoxa genome assembly. (16th May 2019)

• Record Type: Journal Article
• Authors: Price, Dana C; Goodenough, Ursula W; Roth, Robyn; Lee, Jae-Hyeok; Kariyawasam, Thamali; Mutwil, Marek; Ferrari, Camilla; Facchinelli, Fabio; Ball, Steven G; Cenci, Ugo; Chan, Cheong Xin; Wagner, Nicole E; Yoon, Hwan Su; Weber, Andreas P M; Bhattacharya, Debashish
• Is Part Of: DNA research. Volume 26:Number 4(2019)
• Page Start: 287
• Page End: 299
• Publication Date: 2019-05-16
• Subjects: Cyanophora paradoxa -- Archaeplastida -- phylogenomics -- tree of eukaryotes -- ultrastructure; Genes -- Periodicals; Genomes -- Periodicals; Genes -- Research -- Periodicals; Genomics -- Periodicals; DNA -- Research -- Periodicals; 572.86072
• DOI: 10.1093/dnares/dsz009
• Languages: English
• ISSNs: 1340-2838

Abstract

Abstract: Glaucophyta are members of the Archaeplastida, the founding group of photosynthetic eukaryotes that also includes red algae (Rhodophyta), green algae, and plants (Viridiplantae). Here we present a high-quality assembly, built using long-read sequences, of the ca. 100 Mb nuclear genome of the model glaucophyte Cyanophora paradoxa . We also conducted a quick-freeze deep-etch electron microscopy (QFDEEM) analysis of C. paradoxa cells to investigate glaucophyte morphology in comparison to other organisms. Using the genome data, we generated a resolved 115-taxon eukaryotic tree of life that includes a well-supported, monophyletic Archaeplastida. Analysis of muroplast peptidoglycan (PG) ultrastructure using QFDEEM shows that PG is most dense at the cleavage-furrow. Analysis of the chlamydial contribution to glaucophytes and other Archaeplastida shows that these foreign sequences likely played a key role in anaerobic glycolysis in primordial algae to alleviate ATP starvation under night-time hypoxia. The robust genome assembly of C. paradoxa significantly advances knowledge about this model species and provides a reference for exploring the panoply of traits associated with the anciently diverged glaucophyte lineage.