The Cotton High-Affinity K+ Transporter, GhHAK5a, Is Essential for Shoot Regulation of K+ Uptake in Root under Potassium Deficiency. (14th January 2019)

• Record Type: Journal Article
• Authors: Wang, Yiru; Wang, Ye; Li, Bo; Xiong, Changming; Eneji, A Egrinya; Zhang, Mingcai; Li, Fangjun; Tian, Xiaoli; Li, Zhaohu
• Is Part Of: Plant & cell physiology. Volume 60:Number 4(2019)
• Page Start: 888
• Page End: 899
• Publication Date: 2019-01-14
• Subjects: Cotton -- Grafts -- K+ transporter -- Potassium deficiency -- Virus-induced gene silencing; Plant physiology -- Periodicals; Microbiology -- Periodicals; Cytology -- Periodicals; Cell Physiology -- Periodicals; Plant Physiological Phenomena -- Periodicals; Cytology; Microbiology; Plant physiology; Periodicals; 571.205
• DOI: 10.1093/pcp/pcz003
• Languages: English
• ISSNs: 0032-0781

Abstract

Abstract: Potassium (K) deficiency is a key limiting factor in cotton ( Gossypium hirsutum ) production. By grafting two contrasting cotton cultivars, CCRI41 (more susceptible to K + deficiency) and SCRC22 (more tolerant of K + deficiency), we established that cotton shoot plays a vital role in the regulation of root K + uptake. To identify the genetic basis of this finding, we performed RNA sequencing (RNA-seq) of roots of CCRI41 self-grafts (CCRI41/CCRI41, scion/rootstock) and SCRC22/CCRI41 reciprocal-grafts exposed to K + deficiency. We found that GhHAK5a, an orthologous of Arabidopsis thaliana high-affinity K + transporter, AtHAK5, was significantly induced in the CCRI41 rootstock by the SCRC22 scion. This gene was mainly expressed in roots and was more highly induced by K + deficiency in roots of SCRC22 than those of CCRI41. Agrobacterium-mediated virus-induced gene silencing and yeast complementary assay showed that GhHAK5a is a high-affinity K + uptake transporter. Importantly, silencing of GhHAK5a in the CCRI41 rootstock almost completely inhibited the K + uptake induced by SCRC22 scion in CCRI41 rootstock. We identified a key high-affinity K + transporter, GhHAK5a in cotton, which is the essential target for shoot regulation of root K + uptake under K + deficiency.