Analysis and Comparison of Tissue-Marking Dye Detection via Light Microscopy, Telemicroscopy, and Virtual Microscopy. Issue 1 (17th September 2018)

• Record Type: Journal Article
• Authors: Hon, Jane Date; Chen, Wenjin; Minerowicz, Christine; Thomas, Sumi; Barnard, Nicola; Gilbert, Natalie; Fyfe, Billie
• Is Part Of: American journal of clinical pathology. Volume 151:Issue 1(2019)
• Page Start: 95
• Page End: 99
• Publication Date: 2018-09-17
• Subjects: Tissue-marking dye -- Telemicroscopy -- Virtual microscopy -- Telepathology; Diagnosis, Laboratory -- Periodicals; Pathology -- Periodicals; 616.07
• DOI: 10.1093/ajcp/aqy117
• Languages: English
• ISSNs: 0002-9173

Abstract

Abstract: Objectives: To examine the fidelity of ink color identification using light microscopy (LM), telemicroscopy (TM), and virtual microscopy (VM). Methods: Twenty H&E-stained frozen section slides, prepared after tissue inking with five stain combinations, were assessed by three pathologists using LM, TM, and VM. TM was performed using Mikroscan D2 slide scanner/LiveQ software with various objectives. VM was performed using Mikroscan D2 scanner/Qumulus software, specimens digitized at20×. Results: Sensitivity/specificity by LM was 100%/100% for all colors. TM showed high overall specificity but poor sensitivity, particularly red (54%). VM showed high specificity for all colors except black (69%) and, consequently, poor sensitivity for all colors except black (96%). Conclusions: TMD identification via telepathology showed loss of sensitivity/specificity vs LM and highlighted the need for caution when interpreting TMDs with digital technologies and the need for validation protocols.