Comprehensive analysis of kinase-oriented phospho-signalling pathways. (24th December 2018)

• Record Type: Journal Article
• Authors: Amano, Mutsuki; Nishioka, Tomoki; Tsuboi, Daisuke; Kuroda, Keisuke; Funahashi, Yasuhiro; Yamahashi, Yukie; Kaibuchi, Kozo
• Is Part Of: Journal of biochemistry. Volume 165:Number 4(2019)
• Page Start: 301
• Page End: 307
• Publication Date: 2018-12-24
• Subjects: kinase -- phosphoproteomics -- protein phosphorylation -- signal transduction -- substrate; Biochemistry -- Periodicals; Biochemistry -- Periodicals; Electronic journals; 572.05
• DOI: 10.1093/jb/mvy115
• Languages: English
• ISSNs: 0021-924X

Abstract

Abstract: Accumulating information on eukaryotic protein phosphorylation implies a large and complicated phospho-signalling network in various cellular processes. Although a large number of protein phosphorylation sites have been detected, their physiological consequences and the linkage between each phosphorylation site and the responsible protein kinase remain largely unexplored. To understand kinase-oriented phospho-signalling pathways, we have developed novel substrate screening technologies. In this review, we described the in vitro and in vivo screening methods named kinase-interacting substrate screening analysis and kinase-oriented substrate screening analysis, respectively.