Evaluation of Xpert MTB/RIF Assay for Diagnosis of Tuberculosis in Children. (9th February 2018)

• Record Type: Journal Article
• Authors: Das, Arghya; Anupurba, Shampa; Mishra, Om P; Banerjee, Tuhina; Tripathi, Rajneesh
• Is Part Of: Journal of tropical pediatrics. Volume 65:Number 1(2019:Feb.)
• Page Start: 14
• Page End: 20
• Publication Date: 2018-02-09
• Subjects: childhood TB -- paediatric -- Xpert MTB/RIF -- MGIT -- rifampicin resistance; Pediatrics -- Periodicals; Tropical medicine -- Periodicals; Pediatrics -- Periodicals; Tropical Medicine -- Periodicals; Environmental Health -- Periodicals; Infant; Child; 618.929883
• DOI: 10.1093/tropej/fmy005
• Languages: English
• ISSNs: 0142-6338

Abstract

Abstract: Introduction: Childhood tuberculosis (TB) is now a global priority. With the advent of Xpert MTB/RIF, more TB cases in children are being reported. This study was undertaken to evaluate the performance of Xpert in diagnosis of pulmonary and extra-pulmonary TB in children. Methods: Specimens from 171 suspected TB cases in children aged <15 years were tested with Xpert, culture and smear microscopy in the Department of Microbiology, Institute of Medical Sciences, India. Results: The specimens included 106 gastric aspirates, 51 cerebrospinal fluids, 8 induced sputum and 6 lymph node aspirates. Xpert detected Mycobacterium tuberculosis in 19 cases (14 pulmonary and 5 extra-pulmonary), 7 of which were rifampicin-resistant. Sensitivity, specificity, positive predictive value and negative predictive value of Xpert compared with culture were 88.89, 98.04, 84.21 and 98.68%, respectively. The sensitivity was 100% in children aged 1–5 years and 6–10 years and in gastric aspirates. Conclusion: Xpert is an efficient diagnostic tool in childhood tuberculosis.