Effect of carvacrol essential oils on immune response and inflammation-related genes expression in broilers challenged by lipopolysaccharide. (22nd December 2018)

• Record Type: Journal Article
• Authors: Liu, S D; Song, M H; Yun, W; Lee, J H; Kim, H B; Cho, J H
• Is Part Of: Poultry science. Volume 98:Number 5(2019:May)
• Page Start: 2026
• Page End: 2033
• Publication Date: 2018-12-22
• Subjects: broilers -- carvacrol -- immune responses -- inflammation -- LPS; Poultry -- Periodicals; Poultry -- Periodicals; Poultry Diseases -- Periodicals; Electronic journals; 636.5005
• DOI: 10.3382/ps/pey575
• Languages: English
• ISSNs: 0032-5791

Abstract

ABSTRACT: This experiment was conducted to study the effects of orally administered carvacrol essential oils on immune response and inflammation-related genes expression in broilers challenged by lipopolysaccharide (LPS). Eighty 28-day-old (1.28 ± 0.15 kg) ROSS 308 broilers were assigned to a 2 × 2 factorial arrangement of treatments (20 pens of 1 chick/trt). Factors were carvacrol essential oil (orally administered or non-orally administered) and LPS (challenged or non-challenged). Individually housed broilers were randomly assigned (n = 20 broilers per treatment: 10 males and 10 females) to four treatments: (1) basic diet (CTR), (2) basic diet + carvacrol (CAR), (3) basic diet + LPS-challenge (LPS), (4) basic diet + carvacrol + LPS-challenge (CAR+LPS). All were fed with the same diet. The experimental period was for 15 d, after which injecting LPS significantly up-regulated the gene expression levels of TNF-α ( P < 0.05), IL-1β ( P < 0.05), IL-6 ( P < 0.05), IL-8 ( P < 0.05), TLR2 ( P < 0.05), TLR4 ( P < 0.05), NF-κB p65 ( P < 0.05), AVBD-9 ( P < 0.05), and SIgA( P < 0.05) compared with the CTR group; the broilers were challenged by LPS after oral administration of carvacrol, they had significant lower on the gene expression levels of TNF-α ( P < 0.05), IL-1β ( P < 0.05), IL-6 ( P < 0.05), TLR4 ( P < 0.05), NF-κB p65 ( P < 0.05), and AVBD-9 ( P < 0.05) than the LPS group. In conclusion, the broilers orally administrated carvacrol essential oils inhibited the secretion of inflammatory cytokines caused by LPS, affected the pathway of TLRs/NF-κB, and showed an anti-inflammatory function.