Site-directed spin labelling of proteins by Suzuki–Miyaura coupling via a genetically encoded aryliodide amino acid. Issue 13 (25th January 2019)

• Record Type: Journal Article
• Authors: Kugele, Anandi; Braun, Theresa Sophie; Widder, Pia; Williams, Lara; Schmidt, Moritz Johannes; Summerer, Daniel; Drescher, Malte
• Is Part Of: Chemical communications. Volume 55:Issue 13(2019)
• Page Start: 1923
• Page End: 1926
• Publication Date: 2019-01-25
• Subjects: Chemistry -- Periodicals; 540
• DOI: 10.1039/c8cc09325c
• Languages: English
• ISSNs: 1359-7345

Abstract

Abstract : Kugele et al. report site-directed spin labelling via Suzuki–Miyaura coupling of a nitroxide boronic acid label with the genetically encoded amino acid 4-iodo-l -phenylalanine. Abstract : We report site-directed protein spin labelling via Suzuki–Miyaura coupling of a nitroxide boronic acid label with the genetically encoded amino acid 4-iodo-l -phenylalanine. The resulting spin label bears a rigid biphenyl linkage with lower flexibility than spin label R1. It is suitable to obtain defined electron paramagnetic resonance distance distributions and to report protein–membrane interactions and conformational transitions of α-synuclein.