Effect of Rhus verniciflua Extract on IgE-Antigen-Mediated Allergic Reaction in Rat Basophilic Leukemic RBL-2H3 Mast Cells and Passive Cutaneous Anaphylaxis in Mice. (9th October 2019)

• Record Type: Journal Article
• Authors: Do, Hyun Ju; Hwang, Yeo Jin; Yang, Hye Jin; Park, Kwang Il
• Other Names: Du Caigan Academic Editor.
• Is Part Of: Evidence-based complementary and alternative medicine. Volume 2019(2019)
• Publication Date: 2019-10-09
• Subjects: Alternative medicine -- Periodicals; 615.505
• DOI: 10.1155/2019/6497691
• Languages: English
• ISSNs: 1741-427X

Abstract

Abstract : Rhus verniciflua is widely known for its antioxidant, antibacterial, anticancer, and antiaging efficacy and α -glucosidase inhibition. This study was designed whether Rhus verniciflua extracts inhibit the IgE-antigen-mediated allergic reaction in RBL-2H3 mast cells, and it further investigated the Fc ε RI- and arachidonate-signaling by which Rhus verniciflua extracts exert its antiallergic effects. IgE-antigen-sensitized RBL-2H3 mast cells were investigated for the cytotoxicity of Rhus verniciflua extracts and β -hexosaminidase release, and inflammatory mediators (e.g., TNF- α, IL-4, IL-6, histamine, and PGD2 ) were then assessed. Additionally, we examined expressions of genes involved in arachidonate- and Fc ε RI-signaling pathway in RBL-2H3. Rhus verniciflua extracts inhibited β -hexosaminidase release and production of the inflammatory mediators in RBL-2H3. Rhus verniciflua extracts reduced amounts of histamine and expressions of Fc ε RI signaling-related genes such as Lyn and Syk and phosphorylation of extracellular signal-regulated kinase in mast cells. Finally, in late allergic responses, Rhus verniciflua extracts reduced PGD2 release and COX-2 and cPLA2 phosphorylation expressions from IgE-antigen-mediated mast cells. Lastly, 250–500 mg/kg RVE significantly attenuated the Ag/IgE-induced passive cutaneous anaphylaxis (PCA) reaction in mice. These findings provide novel information on the molecular mechanisms underlying the antiallergy properties of Rhus verniciflua extracts in Fc ɛ RI-mediated allergic reaction.