The solution structure of monomeric CCL5 in complex with a doubly sulfated N‐terminal segment of CCR5. (20th April 2018)

• Record Type: Journal Article
• Authors: Abayev, Meital; Rodrigues, João P. G. L. M.; Srivastava, Gautam; Arshava, Boris; Jaremko, Łukasz; Jaremko, Mariusz; Naider, Fred; Levitt, Michael; Anglister, Jacob
• Is Part Of: FEBS journal. Volume 285:Number 11(2018)
• Page Start: 1988
• Page End: 2003
• Publication Date: 2018-04-20
• Subjects: chemokine receptors -- chemokines -- intermolecular interactions -- NMR -- protein complexes -- proteins -- sulfated tyrosine -- TRNOE; Biochemistry -- Periodicals; Molecular biology -- Periodicals; Pathology, Molecular -- Periodicals; 572
• DOI: 10.1111/febs.14460
• Languages: English
• ISSNs: 1742-464X

Abstract

Abstract : The inflammatory chemokine CCL5, which binds the chemokine receptor CCR5 in a two‐step mechanism so as to activate signaling pathways in hematopoetic cells, plays an important role in immune surveillance, inflammation, and development as well as in several immune system pathologies. The recently published crystal structure of CCR5 bound to a high‐affinity variant of CCL5 lacks the N‐terminal segment of the receptor that is post‐translationally sulfated and is known to be important for high‐affinity binding. Here, we report the NMR solution structure of monomeric CCL5 bound to a synthetic doubly sulfated peptide corresponding to the missing first 27 residues of CCR5. Our structures show that two sulfated tyrosine residues, sY10 and sY14, as well as the unsulfated Y15 form a network of strong interactions with a groove on a surface of CCL5 that is formed from evolutionarily conserved basic and hydrophobic amino acids. We then use our NMR structures, in combination with available crystal data, to create an atomic model of full‐length wild‐type CCR5:CCL5. Our findings reveal the structural determinants involved in the recognition of CCL5 by the CCR5 N terminus. These findings, together with existing structural data, provide a complete structural framework with which to understand the specificity of receptor:chemokine interactions. Database: Structural data are available in the PDB under the accession number6FGP Abstract : The inflammatory chemokine CCL5 binds the chemokine receptor CCR5 in hematopoietic cells, activating signalling pathways with important roles in immune surveillance, inflammation, development and several immune system pathologies. In this work, Meital Abayev and colleagues report the NMR solution structure of a doubly sulfated N‐terminal peptide of CCR5 bound to CCL5. Their findings reveal that two sulfated tyrosines on CCR5 interact with evolutionarily conserved residues on the chemokine. Bioinformatics analyses suggest that such contacts may be conserved across the chemokine family, explaining both the specificity and affinity of receptor:chemokine interactions.